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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis in CEM cell line lysates (35ug/lane).

API5 Antibody

CATALOG NUMBER: 60-149

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This API5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-510 amino acids from the C-terminal region of human API5.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
API5
Additional Names:
Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL, API5
Protein Accession Number:
Q9BZZ5
Protein Gi Number:
353526346
Ncbi Gene Id Number:
8539
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.
Background Reference 1:
Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) :
Background Reference 2:
Ren, K., et al. Pathol. Oncol. Res. 16(2):229-237(2010)
Background Reference 3:
Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009)
Background Reference 4:
Rigou, P., et al. EMBO J. 28(11):1576-1588(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-149

List Size:
400 ul

List Price:

$469.00

Datasheet
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