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Antibody staining APIP in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Raji whole cell lysate Lane 3: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.
Antibody staining APIP in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

APIP Antibody

CATALOG NUMBER: 61-295

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This APIP antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 111-142 amino acids from the Central region of human APIP.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
physical-state:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
APIP
Additional Names:
Methylthioribulose-1-phosphate dehydratase {ECO:0000255, HAMAP-Rule:MF_03116}, MTRu-1-P dehydratase {ECO:0000255, HAMAP-Rule:MF_03116}, 4.2.1.109 {ECO:0000255, HAMAP-Rule:MF_03116}, APAF1-interacting protein {ECO:0000255, HAMAP-Rule:MF_03116}, hAPIP, APIP {ECO:0000255, HAMAP-Rule:MF_03116}
Protein Accession Number:
Q96GX9
Protein Gi Number:
74731866
Ncbi Gene Id Number:
51074
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Catalyzes the dehydration of methylthioribulose-1- phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1- phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.
Background Reference 1:
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Background Reference 2:
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Background Reference 3:
Taylor T.D.,et al.Nature 440:497-500(2006).
Background Reference 4:
Cho D.-H.,et al.J. Biol. Chem. 279:39942-39950(2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-295

List Size:
200 ul

List Price:

$469.00

Datasheet
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