ZIP7 Antibody Cat. No.: 6093

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Anti-ZIP7 antibody (6093) was raised against a peptide corresponding to 17 amino acids near the amino terminus of human ZIP7.

The immunogen is located within amino acids 20-70 of ZIP7.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-1 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
 3) Cat. No. 1204 - K562 Cell Lysate
 4) Cat. No. 1212 - 3T3 (Balb) Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 50kD

Observed: 50kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 1) shows similar ZIP7 expression profile in human cell lines detected by two independent anti-ZIP7 antibodies that recognize different epitopes, 6093 against N-terminus domain and 24-029 against recombinant fusion protein.  ZIP7 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies. 

ISOFORMS: Human ZIP7 has one isoform (469aa, 50kD). Mouse ZIP7 has one isoform (476aa, 51kD) and Rat ZIP7 also has one isoform (468aa, 50kD). 6093 can detect human, mouse and rat.

psi-iconProperties
PURIFICATION:ZIP7 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ZIP7 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ZIP7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:SLC39A7
ALTERNATE NAMES:ZIP7 Antibody: KE4, HKE4, ZIP7, RING5, H2-KE4, D6S115E, D6S2244E, Zinc transporter SLC39A7, Histidine-rich membrane protein Ke4
ACCESSION NO.:NP_001070984
PROTEIN GI NO.:117553619
GENE ID:7922
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ZIP7 Antibody: The zinc transporter ZIP7, also known as SLC39A7, is a member of a family of divalent ion transporters. Zinc is an essential ion for cells and plays significant roles in the growth, development, and differentiation. ZIP7 was initially identified while characterizing genes in the major histocompatibility complex on chromosome 17. ZIP7 mRNA is abundantly and widely expressed and the protein localizes to the Golgi apparatus. It functions to transport intracellular zinc from the Golgi apparatus to the cytoplasm of the cell. ZIP7 expression is expressed by zinc. ZIP7 has been suggested to act a hub for tyrosine kinase activation and may thus be a potential therapeutic target for diseases such as cancer where prevention of tyrosine kinase activation would be advantageous.
REFERENCES:1) Dufner-Beattie et al. J. Biol. Chem.2003; 278:50142-50.
2) Eide. Pflugers Arch.2004; 447:796-800.
3) Taylor and Nicohlson. Biochim. Biophys. Acta.2003; 1611:16-30.
4) Lai et al. Genomics1994; 23:338-42.

ANTIBODIES FOR RESEARCH USE ONLY.

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