ZBED1 Antibody Cat. No.: 5111

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: ZBED1 antibody was raised against a 15 amino acid synthetic peptide from near the center of human ZBED1.

The immunogen is located within amino acids 180 - 230 of ZBED1.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: ZBED1 antibody can be used for detection of ZBED1 by Western blot at 1 μg/mL.

Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
SPECIFICITY: This antibody is predicted to have no cross-reactivity to ZBED2 or ZBED3.
POSITIVE CONTROL:1) Cat. No. 1203 - A549 Cell Lysate

psi-iconProperties
PURIFICATION:ZBED1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ZBED1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ZBED1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ZBED1
ALTERNATE NAMES:ZBED1 Antibody: ALTE, DREF, TRAMP, hDREF, ALTE, KIAA0785, Zinc finger BED domain-containing protein 1, Putative Ac-like transposable element
ACCESSION NO.:AAH15030
PROTEIN GI NO.:15929151
GENE ID:9189
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ZBED1 Antibody: ZBED1, also known as DREF, is the human homolog of the Drosophila protein DREF, a transcriptional regulatory factor required for expression of genes involved in DNA replication and cell proliferation. RNA interference (RNAi) experiments targeting ZBED1 RNA resulted in the inhibition of S phase entry and reduction of histone H1 mRNA in HeLa cells, suggesting that ZBED1 also plays a role in cell proliferation in human cells. At least 22 ribosomal proteins (RPs) possess ZBED1 binding sites in their promoters; anti-ZBED1 RNAi also resulted in decreased RP gene expression. Immunoprecipitation analyses have shown that ZBED1 self-associates in vivo via a carboxyl-terminal hATC domain, and this self-association is required for nuclear localization and DNA binding.
REFERENCES:1) Ohshima N, Takahashi M, and Hirose F. Identification of a human homolog of the DREF transcription factor with a potential role in regulation of the histone H1 gene. J. Biol. Chem.2003; 278:22928-38.
2) Yamashita D, Sano Y, Adachi Y, et al. hDREF regulates cell proliferation and expression of ribosomal protein genes. Mol. Cell. Biol.2007; 27:2003-13.
3) Yamashita D, Komori H, Higuchi Y, et al. Human DNA replication-related element binding factor (hDREF) self-association via hATC domain is necessary for its nuclear accumulation and DNA binding. J. Biol. Chem.2007; 282:7563-75.

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