Datasheet

VTI1b Antibody
CATALOG NUMBER: 8289

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Bovine: (100%)
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:VTI1b antibody can be used for detection of VTI1b by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1201 - HeLa Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide
PREDICTED MOLECULAR WEIGHT:Predicted: 14, 19, 26 kDa

Observed: 27 kDa
SPECIFICITY:VTI1b antibody is human, mouse and rat reactive. At least three isoforms of VTI1a are known to exist; this antibody will detect all three isoforms. VTI1b antibody is predicted to not cross-react with VTI1a.
IMMUNOGEN:VTI1b antibody was raised against a 17 amino acid peptide near the center of human VTI1b.

The immunogen is located within amino acids 150 - 200 of VTI1b.
HOST SPECIES:Rabbit

Properties

PURIFICATION:VTI1b antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:VTI1b antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:VTI1b antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:Vesicle transport through interaction with t-SNAREs 1B, VTI1, VTI1-LIKE, VTI1L, VTI2, v-SNARE, vti1-rp1
ACCESSION NO.:NP_006361
PROTEIN GI NO.:5454166
OFFICIAL SYMBOL:VTI1b
GENE ID:10490

Background

BACKGROUND:Vesicle transport through interaction with t-SNAREs homolog 1 (VTI1a and VTI1b) are involved in vesicular transport from the late endosomes to the trans-Golgi network (1). They are both localized in the trans-Golgi network, with VTI1a also found in the Golgi apparatus and VTI1b in endosomes (2,3). It is thought that VTI1b along with the SNARE protein VAMP8 mediates the fusion of antimicrobial and canonical autophagosomes with lysosomes, an essential process for autophagy (4).
REFERENCES: 1) Fischer VM and Stevens TH. A human homolog can functionally replace the yeast vesicle-associated SNARE Vti1p in two vesicle transport pathways. J. Biol. Chem. 1998; 273:2624-30.
2) Kreykenbohm V, Wenzel D, Antonin W et al. The SNAREs vti1a and vti1b have distinct localization and SNARE complex partners. Eur. J. Cell Biol. 2002; 81:273-80.
3) Antonin W, Riedel D, von Mollard GF, et al. The SNARE Vti1a-beta is localized to small synaptic vesicles and participates in a novel SNARE complex. J. Neurosci. 2000; 20:5724-32.
4) Furuta N, Fujita N, Noda T, et al. Combinational soluble N-ethylmaleimide-sensitive factor attachment protein receptor proteins VAMP8 and Vti1b mediate fusion of antimicrobial and canonical autophagosomes with lysosomes. Mol. Biol. Cell. 2010; 21:1001-10.

For Research Use Only