VISTA Antibody [9E4] Cat. No.: RF16074

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: VISTA antibody was raised against the extracellular domain of human VISTA.
TESTED APPLICATIONS: ELISA, Flow, ICC, IF, IHC-P
APPLICATIONS: VISTA antibody can be used for immunohistochemistry starting at 2 μg/mL and immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 2 μg/mL. Flow cytometry at 10 μg/ml.

Antibody validated: Immunohistochemistry in human samples; Immunocytochemistry in human samples; Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1331 - Human Lymphoma Tissue Lysate
 2) Cat. No. 1306 - Human Spleen Tissue
PREDICTED MOLECULAR WEIGHT: Predicted: 31 kDa

Observed: 47 kDa

psi-iconProperties
PURIFICATION:VISTA Antibody is supplied as protein A purified IgG2b
CLONALITY:Monoclonal
ISOTYPE:IgG2b
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:VISTA Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:VISTA antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:VSIR
ALTERNATE NAMES:VISTA Antibody: VISTA molecule, VSIR, B7-H5, B7H5, GI24, PP2135, SISP1, DD1alpha, VISTA, C10orf54, chromosome 10 open reading frame 54, PD-1H, V-set immunoregulatory receptor, V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation, Chromosome 10 Open Reading Frame 54
ACCESSION NO.:NP_071436
PROTEIN GI NO.:64115
GENE ID:64115
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.
REFERENCES:1) Mayya V., et al . Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46.
2) Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.

ANTIBODIES FOR RESEARCH USE ONLY.

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