SARS-CoV-2 NSP (ORF1b) Reagents

What are ORFs?

ProSci Inc. is committed to COVID-19 research, and continuously provides ORF1a reagents and ORF1b reagents as research tools to scientists worldwide. The entry of SARS-CoV-2 into host cells, from the full-length genomic RNA (29,903 nt) that serves as an mRNA, the first set of viral proteins are translated from the long (>21 kb) open reading frame ORF1ab (1). The ORF1ab-encoded polyprotein is subsequently processed into 16 individual non-structural proteins (NSP) by two proteases PLpro/nsp3 and 3CLpro/nsp5. The 3’ half of ORF1ab, ORF1b, encodes a variety of enzymes critical for viral transcription and replication (1). In addition to the genomic RNA, nine major sub-genomic RNAs are produced (1). ORF1a and ORF1b are also separately translated and, as in all coronaviruses, translation of ORF1b in SARS-CoV-2 requires a programmed -1 ribosomal frameshift (-1 PRF) (2).

The genome RNA is packaged by the structural proteins to assemble progeny virions. Shorter sub-genomic RNAs encode conserved structural proteins spike protein (S), an envelope protein (E), membrane protein (M) and nucleocapsid protein (N), and several accessory proteins. SARS-CoV-2 is known to have at least 6 accessory proteins (ORFs 3a, 7, 7a, 7b, 8, and 10)

ProSci Incorporated offers antibodies and recombinant proteins representing most of the SARS-CoV-2 proteome. Below are antibodies and recombinant proteins for each of the SARS-CoV-2 nonstructural proteins.

References

• (1) D. Kim et al (2020) Cell 181, 125-130
• (2) Y. Sun et al (2021) PNAS 118 No. 26, 20223051118 (https://doi.org/10.1073/pnas. 20223051118)