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SARS-CoV-2 NSP (ORF1a) Reagents

What are ORFs?

ProSci Inc. is committed to COVID-19 research, and continuously provides ORF1a reagents and ORF1b reagents as research tools to scientists worldwide. The entry of SARS-CoV-2 into host cells, from the full-length genomic RNA (29,903 nt) that serves as an mRNA, the first set of viral proteins are translated from the long (>21 kb) open reading frame ORF1ab (1). The ORF1ab-encoded polyprotein is subsequently processed into 16 individual non-structural proteins (NSP) by two proteases PLpro/nsp3 and 3CLpro/nsp5. The 3’ half of ORF1ab, ORF1b, encodes a variety of enzymes critical for viral transcription and replication (1). In addition to the genomic RNA, nine major sub-genomic RNAs are produced (1). ORF1a and ORF1b are also separately translated and, as in all coronaviruses, translation of ORF1b in SARS-CoV-2 requires a programmed -1 ribosomal frameshift (-1 PRF) (2).

The genome RNA is packaged by the structural proteins to assemble progeny virions. Shorter sub-genomic RNAs encode conserved structural proteins spike protein (S), an envelope protein (E), membrane protein (M) and nucleocapsid protein (N), and several accessory proteins. SARS-CoV-2 is known to have at least 6 accessory proteins (ORFs 3a, 7, 7a, 7b, 8, and 10)

ProSci Incorporated offers antibodies and recombinant proteins representing most of the SARS-CoV-2 proteome. Below are antibodies and recombinant proteins for each of the SARS-CoV-2 nonstructural proteins.

ORF1a Reagents

ProSci provides SARS-CoV-2 ORF1a antibodies and recombinant proteins for NSP1 through NSP11:

Antibodies

ProductCat. No.ApplicationClonalitySpecies
SARS-CoV-2 (COVID-19) NSP1 Antibody10-500ELISA, WBPolyclonalRabbit
Antibody to AA 289-518 of SARS214-350IHC, WBPolyclonalRabbit
Antibody to AA 160-188 of SARS2 (NSP1)63-227IHC-P, WBPolyclonalRabbit
Antibody to AA 315-344 of SARS263-228IHC-P, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP2 Antibody (IN)9171ELISAPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP2 Antibody (NT)9173ELISAPolyclonalRabbit
SARS-CoV-2 (COVID-19) PL-Pro (NSP3) Antibody (NT)10-504ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) PL-Pro (NSP3) Antibody (IN1)10-505ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) PL-Pro (NSP3) Antibody (IN2)10-506ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP4 Antibody9175ELISAPolyclonalRabbit
SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) Antibody (IN)9151ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) Antibody (NT)9153ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP6 Antibody9177ELISAPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP7 Antibody9155ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP8 Antibody (IN1)9159ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP8 Antibody (IN2)9167ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP9 Antibody (IN2)9161ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP9 Antibody (IN1)9163ELISA, WBPolyclonalRabbit
SARS-CoV-2 (COVID-19) NSP10 Antibody9179ELISA, WBPolyclonalRabbit

Recombinant Proteins

ProductCat. No.SourceFusion TagSequence
SARS-CoV-2 (COVID-19) NSP1 Recombinant Protein10-416E. coliN-His TagMet1 - Gly180
SARS-CoV-2 (COVID-19) NSP1 Recombinant Protein97-095E. coliC-His TagMet1 - Gly180
SARS-CoV-2 (COVID-19) Papain-like Protease10-119E. coliN-His TagGlu1564 - Lys1878
SARS-CoV-2 NSP1 Recombinant Protein20-218BacteriaN-Term MBP Uncleaved1 - 180
SARS-CoV-2 NSP1 Recombinant Protein20-255BacteriaN-Term GST Uncleaved1 - 180
SARS-CoV-2 (COVID-19) NSP2 Recombinant Protein10-425E. coliN-His TagAla1 - Gly638
SARS-CoV-2 NSP2 Recombinant Protein20-217BacteriaN-Term MBP Uncleaved1 - 638
SARS-CoV-2 NSP2 Recombinant Protein20-254BacteriaN-Term GST Uncleaved1 - 638
SARS-CoV-2 (COVID-19) NSP3 Recombinant Protein10-406E. coliN-His TagGlu1024 - Gln1198
SARS-CoV-2 (COVID-19) PL-Pro Recombinant Protein10-404E. coliN-His TagGlu1564 - Lys1877
SARS-CoV-2 NSP3 Recombinant Protein20-216BacteriaN-Term MBP Uncleaved743 - 1072
SARS-CoV-2 NSP3 Recombinant Protein20-253BacteriaN-Term GST Uncleaved743 - 1072
SARS-CoV-2 (COVID-19) 3C-like Proteinase Recombinant Protein10-400E. coliN-His TagSer1 - Gln306
SARS-CoV-2 NSP5 Recombinant Protein20-187BacteriaN-Term GST Cleaved, C-Term 6His Cleaved1 - 306
SARS-CoV-2 NSP5 Recombinant Protein20-188BacteriaN-Term 6His Uncleaved1 - 306
SARS-CoV-2 NSP5 Recombinant Protein20-215BacteriaN-Term MBP Uncleaved1 - 306
SARS-CoV-2 NSP5 Recombinant Protein20-252BacteriaN-Term GST Uncleaved1 - 306
SARS-CoV-2 (COVID-19) 3C-like Proteinase Recombinant Protein10-412E. coliN-Gst TagSer1 - Thr304
SARS-CoV-2 (COVID-19) 3C-like Proteinase10-116E. coliNT His AviSer1 - Gln306
SARS-CoV-2 (COVID-19) NSP7 Recombinant Protein97-096E. coliC-His TagSer1 - Gln83
SARS-CoV-2 NSP7 Recombinant Protein20-214BacteriaN-Term MBP Uncleaved1 - 83
SARS-CoV-2 NSP7 Recombinant Protein20-251BacteriaN-Term GST Uncleaved1 - 83
SARS-CoV-2 (COVID-19) NSP8 Recombinant Protein10-415E. coliN-His TagAla1 - Gln198
SARS-CoV-2 (COVID-19) NSP8 Recombinant Protein97-097E. coliN-His TagAla1 - Gln198
SARS-CoV-2 NSP8 Recombinant Protein20-213BacteriaN-Term MBP Uncleaved1 - 198
SARS-CoV-2 NSP8 Recombinant Protein20-250BacteriaN-Term GST Uncleaved1 - 198
SARS-CoV-2 (COVID-19) NSP9 Recombinant Protein10-417E. coliN-His TagAsn4141 - Gln4253
SARS-CoV-2 NSP9 Recombinant Protein20-212BacteriaN-Term MBP Uncleaved1 - 198
SARS-CoV-2 NSP9 Recombinant Protein20-249BacteriaN-Term GST Uncleaved1 - 113
SARS-CoV-2 (COVID-19) NSP10 Recombinant Protein10-408E. coliN-His TagAla1 - Gln139
SARS-CoV-2 NSP10 Recombinant Protein20-211BacteriaN-Term MBP Uncleaved1 - 113
SARS-CoV-2 NSP10 Recombinant Protein20-248BacteriaN-Term GST Uncleaved1 - 139
SARS-CoV-2 NSP11 NSP12 Recombinant Protein20-210BacteriaN-Term MBP Uncleaved1 - 139
SARS-CoV-2 NSP11 NSP12 Recombinant Protein20-247BacteriaN-Term GST Uncleaved1 - 932

References

  • (1) Chang et al. J Biomed Sci. 2006 Jan;13(1):59-72.
  • (2) Cui et al. J Virol. 2015 Sep;89(17):9029-43.
  • (3) Chen et al. Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):549-53.
  • (4) Emerg Infect Dis. 2004 Sep;10(9):1558-62.

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