
SARS-CoV-2 NSP (ORF1a) Reagents
What are ORFs?
ProSci Inc. is committed to COVID-19 research, and continuously provides ORF1a reagents and ORF1b reagents as research tools to scientists worldwide. The entry of SARS-CoV-2 into host cells, from the full-length genomic RNA (29,903 nt) that serves as an mRNA, the first set of viral proteins are translated from the long (>21 kb) open reading frame ORF1ab (1). The ORF1ab-encoded polyprotein is subsequently processed into 16 individual non-structural proteins (NSP) by two proteases PLpro/nsp3 and 3CLpro/nsp5. The 3’ half of ORF1ab, ORF1b, encodes a variety of enzymes critical for viral transcription and replication (1). In addition to the genomic RNA, nine major sub-genomic RNAs are produced (1). ORF1a and ORF1b are also separately translated and, as in all coronaviruses, translation of ORF1b in SARS-CoV-2 requires a programmed -1 ribosomal frameshift (-1 PRF) (2).
The genome RNA is packaged by the structural proteins to assemble progeny virions. Shorter sub-genomic RNAs encode conserved structural proteins spike protein (S), an envelope protein (E), membrane protein (M) and nucleocapsid protein (N), and several accessory proteins. SARS-CoV-2 is known to have at least 6 accessory proteins (ORFs 3a, 7, 7a, 7b, 8, and 10)
ProSci Incorporated offers antibodies and recombinant proteins representing most of the SARS-CoV-2 proteome. Below are antibodies and recombinant proteins for each of the SARS-CoV-2 nonstructural proteins.
ORF1a Reagents
ProSci provides SARS-CoV-2 ORF1a antibodies and recombinant proteins for NSP1 through NSP11:
Antibodies
Recombinant Proteins
Product | Cat. No. | Source | Fusion Tag | Sequence |
---|---|---|---|---|
SARS-CoV-2 (COVID-19) NSP1 Recombinant Protein | 10-416 | E. coli | N-His Tag | Met1 - Gly180 |
SARS-CoV-2 (COVID-19) NSP1 Recombinant Protein | 97-095 | E. coli | C-His Tag | Met1 - Gly180 |
SARS-CoV-2 (COVID-19) Papain-like Protease | 10-119 | E. coli | N-His Tag | Glu1564 - Lys1878 |
SARS-CoV-2 NSP1 Recombinant Protein | 20-218 | Bacteria | N-Term MBP Uncleaved | 1 - 180 |
SARS-CoV-2 NSP1 Recombinant Protein | 20-255 | Bacteria | N-Term GST Uncleaved | 1 - 180 |
SARS-CoV-2 (COVID-19) NSP2 Recombinant Protein | 10-425 | E. coli | N-His Tag | Ala1 - Gly638 |
SARS-CoV-2 NSP2 Recombinant Protein | 20-217 | Bacteria | N-Term MBP Uncleaved | 1 - 638 |
SARS-CoV-2 NSP2 Recombinant Protein | 20-254 | Bacteria | N-Term GST Uncleaved | 1 - 638 |
SARS-CoV-2 (COVID-19) NSP3 Recombinant Protein | 10-406 | E. coli | N-His Tag | Glu1024 - Gln1198 |
SARS-CoV-2 (COVID-19) PL-Pro Recombinant Protein | 10-404 | E. coli | N-His Tag | Glu1564 - Lys1877 |
SARS-CoV-2 NSP3 Recombinant Protein | 20-216 | Bacteria | N-Term MBP Uncleaved | 743 - 1072 |
SARS-CoV-2 NSP3 Recombinant Protein | 20-253 | Bacteria | N-Term GST Uncleaved | 743 - 1072 |
SARS-CoV-2 (COVID-19) 3C-like Proteinase Recombinant Protein | 10-400 | E. coli | N-His Tag | Ser1 - Gln306 |
SARS-CoV-2 NSP5 Recombinant Protein | 20-187 | Bacteria | N-Term GST Cleaved, C-Term 6His Cleaved | 1 - 306 |
SARS-CoV-2 NSP5 Recombinant Protein | 20-188 | Bacteria | N-Term 6His Uncleaved | 1 - 306 |
SARS-CoV-2 NSP5 Recombinant Protein | 20-215 | Bacteria | N-Term MBP Uncleaved | 1 - 306 |
SARS-CoV-2 NSP5 Recombinant Protein | 20-252 | Bacteria | N-Term GST Uncleaved | 1 - 306 |
SARS-CoV-2 (COVID-19) 3C-like Proteinase Recombinant Protein | 10-412 | E. coli | N-Gst Tag | Ser1 - Thr304 |
SARS-CoV-2 (COVID-19) 3C-like Proteinase | 10-116 | E. coli | NT His Avi | Ser1 - Gln306 |
SARS-CoV-2 (COVID-19) NSP7 Recombinant Protein | 97-096 | E. coli | C-His Tag | Ser1 - Gln83 |
SARS-CoV-2 NSP7 Recombinant Protein | 20-214 | Bacteria | N-Term MBP Uncleaved | 1 - 83 |
SARS-CoV-2 NSP7 Recombinant Protein | 20-251 | Bacteria | N-Term GST Uncleaved | 1 - 83 |
SARS-CoV-2 (COVID-19) NSP8 Recombinant Protein | 10-415 | E. coli | N-His Tag | Ala1 - Gln198 |
SARS-CoV-2 (COVID-19) NSP8 Recombinant Protein | 97-097 | E. coli | N-His Tag | Ala1 - Gln198 |
SARS-CoV-2 NSP8 Recombinant Protein | 20-213 | Bacteria | N-Term MBP Uncleaved | 1 - 198 |
SARS-CoV-2 NSP8 Recombinant Protein | 20-250 | Bacteria | N-Term GST Uncleaved | 1 - 198 |
SARS-CoV-2 (COVID-19) NSP9 Recombinant Protein | 10-417 | E. coli | N-His Tag | Asn4141 - Gln4253 |
SARS-CoV-2 NSP9 Recombinant Protein | 20-212 | Bacteria | N-Term MBP Uncleaved | 1 - 198 |
SARS-CoV-2 NSP9 Recombinant Protein | 20-249 | Bacteria | N-Term GST Uncleaved | 1 - 113 |
SARS-CoV-2 (COVID-19) NSP10 Recombinant Protein | 10-408 | E. coli | N-His Tag | Ala1 - Gln139 |
SARS-CoV-2 NSP10 Recombinant Protein | 20-211 | Bacteria | N-Term MBP Uncleaved | 1 - 113 |
SARS-CoV-2 NSP10 Recombinant Protein | 20-248 | Bacteria | N-Term GST Uncleaved | 1 - 139 |
SARS-CoV-2 NSP11 NSP12 Recombinant Protein | 20-210 | Bacteria | N-Term MBP Uncleaved | 1 - 139 |
SARS-CoV-2 NSP11 NSP12 Recombinant Protein | 20-247 | Bacteria | N-Term GST Uncleaved | 1 - 932 |
References
- (1) Chang et al. J Biomed Sci. 2006 Jan;13(1):59-72.
- (2) Cui et al. J Virol. 2015 Sep;89(17):9029-43.
- (3) Chen et al. Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):549-53.
- (4) Emerg Infect Dis. 2004 Sep;10(9):1558-62.