VHL Antibody Cat. No.: 45-170

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psi-iconSpecifications
HOST SPECIES:Goat
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Expected Species Reactivity based on sequence homology: Dog
IMMUNOGEN: The immunogen for this antibody is: C-RSLVKPENYRRLD
TESTED APPLICATIONS: EIA, ELISA, IHC, WB
APPLICATIONS: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 17kDa band observed in Human Ovary and Testis lysates and also in lysates of Mouse and Rat Testis and Brain and of all human and mouse cell lines tested (calculated MW of 19.7kDa according to NP_937799.1). Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.Enzyme immunoassay: Sandwich-type ELISA with increasing amount of recombinant VHL captured by a rabbit antibody. Recommended reporter concentration: 1.5-3ug/ml
SPECIFICITY: This antibody is expected to recognise both reported isoforms (NP_000542.1 and NP_937799.1).
POSITIVE CONTROL:1) Cat. No. 1316 - Human Ovary Tissue Lysate

psi-iconProperties
PURIFICATION:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
CONCENTRATION:500 ug/mL
STORAGE CONDITIONS:Aliquot and store at -20˚C. Minimize freezing and thawing.

psi-iconAdditional Info
OFFICIAL SYMBOL:VHL
ALTERNATE NAMES:VHL, von Hippel-Lindau tumor suppressor, HRCA1, RCA1, VHL1 , elongin binding protein, von Hippel-Lindau syndrome
ACCESSION NO.:NP_000542.1, NP_937799.1
PROTEIN GI NO.:4507891
GENE ID:7428
psi-iconBackground and References
REFERENCES:1) Kong X, Lin Z, Liang D, Fath D, Sang N, Caro J. Histone deacetylase inhibitors induce VHL and ubiquitin-independent proteasomal degradation of hypoxia-inducible factor 1alpha. Mol Cell Biol. 2006 Mar;26(6):2019-28.

ANTIBODIES FOR RESEARCH USE ONLY.

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