VEGF Antibody [VEGF/1063] Cat. No.: 33-479

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Dog, Human, Mouse, Rabbit, Rat
IMMUNOGEN: Human recombinant protein was used as the immunogen for the VEGF antibody.
TESTED APPLICATIONS: IF, IHC-P, WB
APPLICATIONS: Immunofluorescence: 1-2 ug/ml

Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)

Optimal dilution of the VEGF antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.


psi-iconProperties
PURIFICATION:Protein G affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG1, kappa
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:VEGFA
ALTERNATE NAMES:Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGFA, VEGF
GENE ID:7422
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.

ANTIBODIES FOR RESEARCH USE ONLY.

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