VASP Antibody [IE273] Cat. No.: 36-170

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Bovine, Human, Pig, Rabbit, Sheep
IMMUNOGEN: Purified human VASP.
TESTED APPLICATIONS: ELISA, ICC, IHC, IP, WB
APPLICATIONS: Immunocytochemistry: (1-5ug/ml, 1:100-1:500) . Western Blot: (0.01-0.25ug/ml, 1:2000-1:50,000). Optimal conditions must be determined individually for each application.
SPECIFICITY: Recognizes both the 46kDa (Ser157 dephospho-) and 50kDa (Ser157 phospho-) form of human, porcine, bovine, sheep and rabbit VASP.

psi-iconProperties
PURIFICATION:Protein A/G purified.
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Liquid. In PBS containing 2mg/ml BSA and 0.02% sodium azide.
CONCENTRATION:500 ug/ml
STORAGE CONDITIONS:Stable for at least 1 year after receipt when stored at -20˚C.

psi-iconAdditional Info
OFFICIAL SYMBOL:VASP
ALTERNATE NAMES:Vasodilator-stimulated Phosphoprotein
ACCESSION NO.:NP_003361.1
PROTEIN GI NO.:4507869
GENE ID:7408
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:VASP (vasodilator-stimulated phosphoprotein) is a proline-rich protein substrate of cAMP- and cGMP-dependent protein kinases. VASP is an actin-associated protein involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping proteins. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments and it plays a role in actin-based mobility of Listeria monocytogenes in host cells. It regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation. VASP phosphorylation is used to monitor the effect of so-called antiplatelet drugs that reduce platelet reactivity and are used to prevent stent thrombosis, strokes and heart attacks in patients. Phosphorylation of VASP at Ser157 causes a mobility shift in SDS gel electrophoresis from 46 to 50kDa, which has been used as a convenient marker to monitor cyclic nucleotide-dependent protein kinase activity.

ANTIBODIES FOR RESEARCH USE ONLY.

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