UCHL1 Recombinant Protein Cat. No.: 96-762

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psi-iconSpecifications
SPECIES:Human
SOURCE SPECIES:E. coli
SEQUENCE:Met 1 - Ala 223
FUSION TAG:His Tag
TESTED APPLICATIONS:WB
APPLICATIONS:This recombinant protein can be used for WB. For research use only.
psi-iconProperties
PURITY:>92% as determined by SDS-PAGE.
PREDICTED MOLECULAR WEIGHT:25.7 kDa
PHYSICAL STATE:Lyophilized
BUFFER:20mM Tris, 500mM Nacl,1mM DTT,pH 8.0
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -71˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:UCHL1, PGP9.5
ACCESSION NO.:NP_004172
OFFICIAL SYMBOL:UCHL1
GENE ID:7345
psi-iconBackground and References
BACKGROUND:Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ,a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain. UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also,UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity. Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.
REFERENCES:1) Choi J., et al., 2004, J. Biol. Chem. 279:13256-13264.
2) Liu Y., et al., 2002, Cell 111:209-218.
3) Choudhary C., et al., 2009, Science 325:834-840.

FOR RESEARCH USE ONLY.

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