TRPV4 Antibody Cat. No.: 7695

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
IMMUNOGEN: TRPV4 antibody was raised against an 18 amino acid peptide near the center of human TRPV4.

The immunogen is located within amino acids 380 - 430 of TRPV4.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: TRPV4 antibody can be used for detection of TRPV4 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 5 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
 2) Cat. No. 10-101 - Human Lung Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 96 kDa

Observed: 100 kDa

psi-iconProperties
PURIFICATION:TRPV4 antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:TRPV4 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TRPV4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:TRPV4
ALTERNATE NAMES:TRPV4 Antibody: SMAL, VRL2, CMT2C, SPSMA, TRP12, VROAC, HMSN2C, OTRPC4, SSQTL1, Transient receptor potential cation channel subfamily V member 4, Osm-9-like TRP channel 4, TrpV4
ACCESSION NO.:NP_067638
PROTEIN GI NO.:22547184
GENE ID:59341
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).
REFERENCES:1) Birnbaumer L, Yildirim E and Abramowitz J. A comparison of the genes coding for canonical TRP channels and their M, V and P relatives. Cell Calcium 2003; 33:419-32.
2) Alessandri-Haber N, Dina OA, Yeh JJ, et al. Transient receptor potential vanilloid 4 is essential in chemotherapy-induced neuropathic pain in the rat. J. Neurosci. 2004; 24:4444-52.
3) Liedtke W. TRPV4 plays an evolutionary conserved role in the transduction of osmotic and mechanical stimuli in live animals. J. Physiol. 2005; 567:53-8.
4) Shibasaki K, Suzuki M, Mizuno A, et al. Effects of body temperature on neural activity in the hippocampus: regulation of resting membrane potentials by transient receptor potential vanilloid 4. J. Neurosci. 2007; 27:1566–75.

ANTIBODIES FOR RESEARCH USE ONLY.

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