Trophinin Antibody Cat. No.: 8245

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Trophinin antibody was raised against a 16 amino acid peptide near the amino terminus of human Trophinin.

The immunogen is located within amino acids 390 - 440 of Trophinin.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: Trophinin antibody can be used for detection of Trophinin by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry at 10 μg/ml.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: Trophinin antibody is human, mouse and rat reactive. Multiple isoforms of Trophinin are known to exist.
POSITIVE CONTROL:1) Cat. No. 1464 - Rat Liver Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 78, 157 kDa

Observed: 94 kDa

psi-iconProperties
PURIFICATION:Trophinin antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Trophinin antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:Trophinin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:TRO
ALTERNATE NAMES:TRO, MAGE-d3, MAGED3
ACCESSION NO.:NP_001034794
PROTEIN GI NO.:89276766
GENE ID:7216
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Trophinin, also known as TRO or MAGED3, is an apical cell adhesion molecule that is implicated in the initial attachment during the process of embryo implantation and functions by mediating cell adhesion between trophoblastic and endometrial epithelial cells (1,2). Trophinin is a membrane protein expressed in chorionic villi trophoblasts and in maternal endometrial epithelial cells in an implantation-dependent manner and interacts with Bystin and Tastin, facilitating cell adhesion and embryo implantation (3,4). The induction of Trophinin expression may be a useful method for improving implantation rates and can be a potential diagnostic factor and biomarker for human cancer (4,5).
REFERENCES:1) Fukuda MN, Sato T, Nakayama J, et al. Trophinin and tastin, a novel cell adhesion molecule complex with potential involvement in embryo implantation. Genes Dev. 1995; 9:1199-210.
2) Ma L, Yin M, Wu X, et al. Expression of trophinin and bystin identifies distinct cell types in the germinal zones of adult rat brain. Eur. J. Neurosci. 2006; 23:2265-76.
3) Tamura N, Sugihara K, Akama TO, et al. Trophinin-mediated cell adhesion induces apoptosis of human endometrial epithelial cells through PKC-d. Cell Cycle 2011; 10:135-43.
4) Chen KY, Lee YC, Lai JM, et al. Identification of trophinin as an enhancer for cell invasion and a prognostic factor for early stage lung cancer. Eur. J. Cancer. 2007; 43:782-90.

ANTIBODIES FOR RESEARCH USE ONLY.

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