Trim5a Antibody [3B11H2] Cat. No.: 32-231

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: Ni-NTA purified truncated recombinant human trim5 alpha expressed in E. Coli strain BL21 (DE3).
TESTED APPLICATIONS: IHC, WB
APPLICATIONS: Western Blot:Dilution 1:1,000 - 1:5,000
IHC(P):Dilution 1:100 - 1:400
IHC(F):Dilution 1:200 - 1:400
Determining optimal working dilutions by titration test.

psi-iconProperties
CLONALITY:Monoclonal
ISOTYPE:IgG1
CONJUGATE:Unconjugated
BUFFER:Ascitic fluid containing 0.03% sodium azide.
STORAGE CONDITIONS:Trim5α monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ZCCHC3
ALTERNATE NAMES:C20orf99C20orf99
ACCESSION NO.:Q9NUD5
PROTEIN GI NO.:23396534
GENE ID:85364
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:TRIM5-alpha is a protein that is found in the cells of many mammals and fends of various retrovirus infections. It protects monkeys from infection with HIV-1, and humans from infection with some other viruses. If a retrovirus has entered a cell, it needs to shed its capsid in order to reversely transcribe its genes, so that they can be expressed by the host cell. It is believed that TRIM5α, which is present in the cytoplasm, somehow recognizes the capsid and blocks its shedding, thereby stopping the virus in its tracks. It thus represents an intracellular defense completely separate from the rest of the body's immune system.
REFERENCES:1) Stremlau, M. Nature 2004.427:848-53.
2) Song, B. J Virol. 2005.79(7):3930-7.

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