TRIM58 Antibody Cat. No.: 8545

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: TRIM58 antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TRIM58.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: TRIM58 antibody can be used for Western blot at 1 - 2 μg/mL.

Antibody validated: Western Blot in human and mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1301 - Human Heart Tissue Lysate
 2) Cat. No. 1405 - Mouse Kidney Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 53 kDa

Observed: 56 kDa

psi-iconProperties
PURIFICATION:TRIM58 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:TRIM58 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TRIM58 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:TRIM58
ALTERNATE NAMES:TRIM58 Antibody: Tripartite motif containing 58 protein, BIA2
ACCESSION NO.:NP_056246
PROTEIN GI NO.:112421127
GENE ID:25893
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:TRIM58 Antibody: TRIM58 is a member of the tripartite motif-containing family of proteins that frequently possess E3 ubiquitin ligase activities (1). TRIM58 is strongly expressed in erythroid lineage cells and is further upregulated during late erythroid maturation. Recent experiments suggest that TRIM58 facilitates erythroblast enucleation by ubiquitinating the intermediate chain of the microtubule motor dynein, thereby inducing proteolytic degradation (2).
REFERENCES:1) Napolitano LM and Meroni G. TRIM family: Pleiotropy and diversification through homomultimer and heteromultimer formation. IUBMB Life 2012; 64:64-71.
2) Thom CS, Traxler EA, Khandros E, et al. Trim58 degrades dynein and regulates terminal erythropoiesis. Dev. Cell 2014; 30:688-700.

ANTIBODIES FOR RESEARCH USE ONLY.

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