TNFAIP1 Antibody Cat. No.: 8345

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (100%)
IMMUNOGEN: TNFAIP1 antibody was raised against a 19 amino acid peptide from near the carboxy terminus of human TNFAIP1.

The immunogen is located within the last 50 amino acids of TNFAIP1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: TNFAIP1 antibody can be used for the detection of TNFAIP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 10 μg/ml.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: TNFAIP1 antibody is human, mouse and rat reactive. TNFAIP1 antibody is predicted to not cross-react with other TNFAIP proteins.
POSITIVE CONTROL:1) Cat. No. 1403 - Mouse Brain Tissue Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 35 kDa

Observed: 35 kDa

psi-iconProperties
PURIFICATION:TNFAIP1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:TNFAIP1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TNFAIP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:TNFAIP1
ALTERNATE NAMES:Tumor necrosis factor, alpha-induced protein 1, BTB/POZ domain-containing protein TNFAIP1,BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2, BACURD2, EDP1
ACCESSION NO.:NP_066960
PROTEIN GI NO.:10863937
GENE ID:7126
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:TNFAIP1 is a BTB/POZ domain-containing protein that belongs to the KCTD10/BACURD family which mediates POZ-POZ interactions and chromatin modeling (1,2). The expression of TNFAIP1 is developmentally regulated in a tissue-specific manner and can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells (3). TNFAIP1 may be involved in DNA repair, DNA synthesis, and cell apoptosis. TNFAIP1 is suggested to play a role in the process of cancer and in the innate immunity against the Hepatitis B virus (4,5).
REFERENCES:1) Liu M, Sun Z, Zhou A, et al. Functional characterization of the promoter region of human TNFAIP1 gene. Mol. Biol. Rep. 2010; 37:1699-705.
2) Hu X, Yan F, Wang F, et al. TNFAIP1 interacts with KCTD10 to promote the degradation of KCTD10 proteins and inhibit the transcriptional activities of NF-?B and AP-1. Mol. Biol. Rep. 2012; 39:9911-9.
3) Zhang CL, Wang C, Yan WJ, et al. Knockdown of TNFAIP1 inhibits growth and induces apoptosis in osteosarcoma cells through inhibition of the nuclear factor-?B pathway. Oncol. Rep. 2014; 32:1149-55.
4) Kim DM, Chung KS, Choi SJ, et al. RhoB induces apoptosis via direct interaction with TNFAIP1 in HeLa cells. Int. J. Cancer 2009; 125:2520-7.

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