TMEM59L Antibody Cat. No.: 6435

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (89%)
IMMUNOGEN: TMEM59L antibody was raised against a 19 amino acid synthetic peptide near carboxy terminus of human TMEM59L.

The immunogen is located within amino acids 220 - 270 of TMEM59L.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: TMEM59L antibody can be used for detection of TMEM59L by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: Multiple isoforms of TMEM59L are known to exist. TMEM59L antibody is predicted to not cross-react TMEM59.
POSITIVE CONTROL:1) Cat. No. 1461 - Rat Heart Tissue Lysate

psi-iconProperties
PURIFICATION:TMEM59L Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:TMEM59L Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TMEM59L antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:TMEM59L
ALTERNATE NAMES:TMEM59L Antibody: BSMAP, C19orf4, BSMAP, Transmembrane protein 59-like, Brain-specific membrane-anchored protein
ACCESSION NO.:NP_036241
PROTEIN GI NO.:6912274
GENE ID:25789
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:TMEM59L Antibody: Processing of the amyloid precursor protein (APP) by two different proteases, called alpha- and beta-secretase, is a central regulatory event in the generation of the amyloid beta peptide (Abeta), which has a key role in Alzheimer disease (AD) pathogenesis. TMEM59L is a type I membrane glycoprotein that is expressed in the brain. A related protein, TMEM59, is a Golgi-localized protein that modulates the O-glycosylation and complex N-glycosylation steps occurring during the Golgi maturation of several proteins such as APP, BACE1, SEAP or PRNP and inhibits APP transport and shedding.
REFERENCES:1) Schöbel S, Neumann S, Seed B, et al. Expression cloning screen for modifiers of amyloid precursor protein shedding. Int. J. Dev. Neurosci. 2006; 24:141-8.
2) Schöbel S, Neumann S, Hertweck M, et al. A novel sorting nexin modulates endocytic trafficking and alpha-secretase cleavage of the amyloid precursor protein. J. Biol. Chem. 2008; 283:14257-68.
3) Elson GC, de Coignac AB, Aubry JP, et al. BSMAP, a novel protein expressed specifically in the brain whose gene is localized on chromosome 19p12. Biochem. Biophys. Res. Commun. 1999; 264:55-62.
4) Ullrich S, M√ľnch A, Neumann S, et al. The novel membrane protein TMEM59 modulates complex glycosylation, cell surface expression, and secretion of the amyloid precursor protein. J. Biol. Chem. 2010; 285:20664-74.

ANTIBODIES FOR RESEARCH USE ONLY.

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