TIP47 Antibody Cat. No.: 3881

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (86%)
IMMUNOGEN: Anti-TIP47 antibody (3881) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TIP47.

The immunogen is located within the last 50 amino acids of TIP47.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-4 μg/mL; IHC-P: 10 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and rat samples; Immunohistochemistry in rat samples; Immunofluorescence in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1224 - Daudi Cell Lysate
 2) Cat. No. 1464 - Rat Liver Tissue Lysate
 3) Cat. No.1204 - K562 Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 47kD

Observed: 47kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIP47 expression profile in human cell lines detected by two independent anti-TIP47 antibodies that recognize different epitopes, 3883 against N-terminus domain and 3881 against C-terminus domain.  TIP47 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Induced Expression Validation (Figure 6-8): TIP47 expression detected by anit-TIP47 antibodies was increased in Atg7 liver KO mice (figure 6), after starvation (figure 7), or after oleic acid treatment (figure 8).

ISOFORMS: Human TIP47 has 4 isoforms, including isoform 1 (434aa, 47kD), isoform 2 (251aa, 28kD), isoform 3 (433aa, 47kD), isoform 4 (422aa, 46kD). Mouse TIP47 has one isoform (437aa, 47kD) and rat TIP47 also has one isoform (438aa, 47kD). 3883 can detect all human isoforms, and can also detect mouse and rat TIP47.

psi-iconProperties
PURIFICATION:TIP47 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:TIP47 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TIP47 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:PLIN3
ALTERNATE NAMES:TIP47 Antibody: PP17, TIP47, M6PRBP1, Perilipin-3, 47 kDa mannose 6-phosphate receptor-binding protein, 47 kDa MPR-binding protein
ACCESSION NO.:O60664
PROTEIN GI NO.:68846601
GENE ID:10226
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:TIP47 Antibody: Tail-interacting protein (TIP47) is a cytosolic protein essential for the transport of mannose-6-phosphate receptors (MPRs) from endosomes to the trans-Golgi compartments in cells. TIP47 is recruited from the cytoplasm to the late endosomal surface by the Ras-associated protein Rab9 GTPase, enabling it to bind more efficiently to MPR cytoplasmic domains. Recently, it was shown that TIP47 regulates the expression of Rab9, as expression of siRNA to TIP47 in transfected cells dramatically decreased the half-life of the Rab9 protein in addition to stabilizing the subcellular localization of Rab9. At least two isoforms of TIP47 are known to exist.
REFERENCES:1) Barbero et al. J. Cell Biol. 2002; 156:511-8.
2) Carroll et al. Science 2001; 292:1373-7.
3) Aivazian et al. J. Cell Biol. 2006; 173:917-26.
4) Ganley et al. Mol. Biol. Cell 2004; 15:5420-30.
psi-iconCITATIONS
CITATIONS: 1)Singh et al. Autophagy regulates lipid metabolism. Nature. 2009;458(7242):1131-5. PMID: 19339967
2)Qi et al. FOXO3 growth inhibition of colonic cells is dependent on intraepithelial lipid droplet density. J Biol Chem. 2013;288(23):16274-81. PMID: 23603907

ANTIBODIES FOR RESEARCH USE ONLY.

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