TIM-1 Antibody Cat. No.: 3809

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (63%), Rat: (56%)
IMMUNOGEN: Anti-TIM-1 antibody (3809) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1.

The immunogen is located within amino acids 50 - 100 of TIM-1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 1 - 8 μ g/mL (overnight incubation at 4˚ C); IHC-P: 10 μ g/mL; IF: 20 μ g/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1202 - A431 Cell Lysate
 2) Cat. No. 1203 - A549 Cell Lysate
 3) Cat. No. 1224 - Daudi Cell Lysate
 4) Cat. No. 11-401 - Human Uterus Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 44kD

Observed: 60kD (Post-modification: 4 N-linked glycosylation)
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TIM-1 expression profile in human cell lines detected by two independent anti-TIM-1 antibodies that recognize different epitopes, 3809 against N-terminus domain and 3811 against the center ectodomain.  TIM-1 proteins are detected in all the tested cell lines except MCF-7 at different expression levels by the two independent antibodies. 

siRNA knockdown validation (Figure 3): Anti-TIM-1 antibody (3809) specificity was further verified by TIM-1 specific siRNA knockdown. TIM-1 signal in HeLa cells transfected with TIM-1 siRNAs was much weaker in comparison with that in HeLa cells transfected with control siRNAs.

ISOFORMS: Human TIM-1 has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD). This antibody detects human isoform b precursor. Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1 has only one isoform identified so far (307aa, 34kD).

psi-iconProperties
PURIFICATION:TIM-1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TIM-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:HAVCR1
ALTERNATE NAMES:TIM-1 Antibody: TIM, KIM1, TIM1, HAVCR, KIM-1, TIM-1, TIMD1, TIMD-1, HAVCR-1, Hepatitis A virus cellular receptor 1, Kidney injury molecule 1, HAVcr-1
ACCESSION NO.:NP_036338
PROTEIN GI NO.:153085427
GENE ID:26762
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
REFERENCES:1) Feigelstock et al. J. Virol. 1998; 72:6621-8
2) Ichimura et al. J. Biol. Chem. 1998; 273:4135-42
3) Meyers et al. Mol. Med. 2005; 11:362-9
4) Mayers et al. Nat. Immunol. 2005;6:455-64.

ANTIBODIES FOR RESEARCH USE ONLY.

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