TIGIT Recombinant Protein Cat. No.: 96-799

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psi-iconSpecifications
SPECIES:Cynomolgus, Rhesus macaque
SOURCE SPECIES:HEK293 cells
SEQUENCE:Met 89 - Ile 209
FUSION TAG:Fc Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This recombinant protein can be used for E, WB. For research use only.
BIOLOGICAL ACTIVITY: Measured by its binding ability in a functional ELISA. Immobilized Human CD155, His Tag at 10 ug/mL (100 uL/well) can bind Cynomolgus / Rhesus macaque TIGIT, Fc Tag with a linear range of 2.22-20 ug/mL (QC tested).
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.

Endotoxin level is less than 1.0 EU per ug by the LAL method.
PREDICTED MOLECULAR WEIGHT:40 kDa
PHYSICAL STATE:Lyophilized
BUFFER:50 mM Tris, 100 mM Glycine, pH7.5
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:TIGIT,VSIG9,VSTM3
ACCESSION NO.:G7NXM4
OFFICIAL SYMBOL:TIGIT
psi-iconBackground and References
BACKGROUND:T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is also known as V-set and immunoglobulin domain-containing protein 9 (VSIG9), V-set and transmembrane domain-containing protein 3 (VSTM3),which belongs to single-pass type I membrane protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level). TIGIT binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
REFERENCES:1) Yu X., et al., 2009, Nat. Immunol. 10:48-57.
2) Stengel K.F., et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109:5399-5404.
3) Lozano E., et al., 2012, J. Immunol. 188:3869-3875.

FOR RESEARCH USE ONLY.

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