TEM1 Antibody Cat. No.: 4357

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse (86%); Rat: (86%)
IMMUNOGEN: Anti-TEM-1 antibody (4357) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1.

The immunogen is located within the last 50 amino acids of TEM-1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.

Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.
POSITIVE CONTROL:1) Cat. No. 1320 - Human Colon Tissue Lysate
 2) Cat. No. 1223 - Caco-2 Cell Lysate
 3) Cat. No. 1219 – MCF-7 Cell Lysate
 4) Cat. No. 1220 – SK-N-SH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 81kD and 46kD

Observed: 90 kD (post-modifications: 27 O-linked glycosylations) and 46kD
psi-iconAdvanced Validation
VALIDATION:

Independent Antibody Validation in Cell lines (Figure 2) shows similar TEM-1 expression profile in human and mouse cell lines detected by two independent anti-TEM-1 antibodies that recognize different epitopes, 4357 against C-terminus domain and 4359 against N-terminus domain.  TEM-1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

ISOFORMS: Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD). 4357 can detect all isoforms of human, mouse and rat.

psi-iconProperties
PURIFICATION:TEM1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:TEM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:CD248
ALTERNATE NAMES:TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1
ACCESSION NO.:NP_065137
PROTEIN GI NO.:9966885
GENE ID:57124
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.
REFERENCES:1) Rettig et al. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6.
2) Christian et al. J. Biol. Chem. 2001; 276:7408-14.
3) Nanda et al. Curr. Opin. Oncol. 2004; 16:44-9.
4) Nanda et al. Proc. Natl. Acad. Sci. USA 2006; 103:3351-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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