TDG Antibody Cat. No.: 62-366

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: This TDG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-310 amino acids from the C-terminal region of human TDG.
TESTED APPLICATIONS: Flow, WB
APPLICATIONS: For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
PREDICTED MOLECULAR WEIGHT: 46 kDa

psi-iconProperties
PURIFICATION:This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
CLONALITY:Polyclonal
ISOTYPE:Rabbit Ig
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Supplied in PBS with 0.09% (W/V) sodium azide.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:TDG
ALTERNATE NAMES:G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase, hTDG, TDG
ACCESSION NO.:Q13569
PROTEIN GI NO.:46397791
GENE ID:6996
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The protein TDG belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine.
REFERENCES:1) Kim,E.J., Biochem. Biophys. Res. Commun. 377 (3), 838-842 (2008)
2) Neddermann,P., J. Biol. Chem. 271 (22), 12767-12774 (1996)

ANTIBODIES FOR RESEARCH USE ONLY.

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