SYNPO2 Antibody Cat. No.: 6051

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
IMMUNOGEN: SYNPO2 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human SYNPO2.

The immunogen is located within amino acids 320 - 370 of SYNPO2.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: SYNPO2 antibody can be used for detection of SYNPO2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate

psi-iconProperties
PURIFICATION:SYNPO2 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SYNPO2 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SYNPO2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:SYNPO2
ALTERNATE NAMES:SYNPO2 Antibody: Synaptopodin-2, Genethonin-2
ACCESSION NO.:NP_597734
PROTEIN GI NO.:193083183
GENE ID:171024
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SYNPO2 Antibody: SYNPO2 was initially identified as myopodin, a member of the synaptopodin family that contains one PPXY motif and multiple PXXP motifs. It colocalizes with alpha-actinin and is found at the Z-disc and during stress conditions will translocate to the nucleus, suggesting that it is part of signaling pathways in addition to its function as a structural protein. SYNPO2 has been shown to bind to calmodulin, alpha-actinin, and smooth muscle myosin and will stimulate actin polymerization in a calmodulin dependent manner, consistent with its proposed role in organizing the cytoskeleton. While deletion of SYNPO2 has been reported to be highly correlated with the invasiveness of prostate cancers, other reports suggest that down-regulation of SYNPO2 reduces the invasiveness and motility of prostate cancer cells.
REFERENCES:1) Weins A, Schwarz K, Faul C, et al. Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein. J. Cell Biol.2001; 155:393-404.
2) Shroeter MM, Beall B, Heid HW, et al. In vitro characterization of native mammalian smooth-muscle protein synaptopodin. Biosci. Rep.2008; 195-203.
3) Lin F, Yu YP, Woods J, et al. Myopodin, a synaptopodin homologue, is frequently deleted in invasive prostate cancers. Am. J. Path.2001; 5:1603-12.
4) De Ganck A, De Corte V, Bruyneel E, et al. Down-regulation of myopodin expression reduces invasion and motility of PC-3 prostate cancer cells. Int. J. Oncol.2009; 34:1403-9.

ANTIBODIES FOR RESEARCH USE ONLY.

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