StrepII-tag Antibody Cat. No.: 4335

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Tag
IMMUNOGEN: StrepII-tag antibody was raised against the amino acid sequence "srWSHPQFEKsh".
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: StrepII-tag antibody can be used for the detection of StrepII by Western blot at 1 μg/mL.

psi-iconProperties
PURIFICATION:StrepII-tag Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:StrepII-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
ALTERNATE NAMES:StrepII-tag Antibody:
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
REFERENCES:1) Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22.
2) Schmidt TGM, Koepe J, Frank R, et al. Molecular interactions between the Strep-tag affinity peptide and its cognate target, streptavidin. J. Mol. Biol.1996; 255:753-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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