StrepII-tag Antibody Cat. No.: 4335

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Tag
IMMUNOGEN: Anti-StrepII-tag antibody (4335) StrepII-tag antibody was raised against the amino acid sequence " srWSHPQFEKsh".
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: WB: 0.25-1 μg/mL

Antibody validated: StrepII-tag antibody can be used for the detection of StrepII-tagged proteins
psi-iconAdvanced Validation
VALIDATION:

Recombinant Protein Test (Figure 1&2): Anti-StrepII-tag antibodies (4335) detected StrepII-tagged recombinant protein at different concentrations.


psi-iconProperties
PURIFICATION:StrepII-tag Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:StrepII-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
ALTERNATE NAMES:StrepII-tag Antibody:
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
REFERENCES:1) Schmidt and SKerra. Protein Eng.1993;6:109-22.
2) Schmidt et al. J. Mol. Biol. 1996;255:753-6.

ANTIBODIES FOR RESEARCH USE ONLY.

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