SRPX1 Antibody Cat. No.: 5473

Availability: In stock

$0.00

 Price Options

$0.00
psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: SRPX1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human SRPX1.

The immunogen is located within amino acids 30 - 80 of SRPX1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: SRPX1 antibody can be used for detection of SRPX1 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1220 - SK-N-SH Cell Lysate
 2) Cat. No. 10-301 - Human Brain Tissue Slide

psi-iconProperties
PURIFICATION:SRPX1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SRPX1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SRPX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:SRPX
ALTERNATE NAMES:SRPX1 Antibody: DRS, ETX1, SRPX1, HEL-S-83p, Sushi repeat-containing protein SRPX
ACCESSION NO.:NP_006298
PROTEIN GI NO.:5454086
GENE ID:8406
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SRPX1 Antibody: SRPX1 was initially identified as a candidate gene for X-linked retinitis pigmentosa and is overexpressed in the trabecular network of the eye in glaucoma conditions. Its expression is significantly downregulated in a number cancer cell lines and malignant tumor tissues and its ectopic expression induces apoptosis through the endoplasmic reticulum and the activation of caspase-12, -9, and -3, indicating that it can function as a tumor suppressor. SRPX1 is also involved with the regulation of autophagy under low serum conditions and can associate with Rab24, a member of the Rab GTPase protein family that is involved in autophagy. This association of SRPX1 with Rab24 is enhanced during autophagy.
REFERENCES:1) Dry KL, Aldred MA, Edgar AJ, et al. Identification of a novel gene, ETX1 from Xp21.1, a candidate gene for X-linked retinitis pigmentosa (RP3). Hum. Mol. Genet.1995; 4:2347-53.
2) Iragavarapu S, Algeciras ME, Lee RK, et al. ETX1 is over-expressed in the glaucomatous trabecular meshwork. Mol. Vision2009; 15:2061-7.
3) Shimakage M, Kawahara K, Kikkawa N, et al. Downregulation of drs mRNA in human colon adenocarcinomas. Int. J. Cancer2000; 87:5-11.
4) Tambe Y, Isono T, Haraguchi S, et al. A novel apoptotic pathway induced by the drs tumor suppressor gene. Oncogene2004; 23:2977-87.

ANTIBODIES FOR RESEARCH USE ONLY.

For additional information, visit ProSci's Terms & Conditions Page.