SRPK1 Antibody Cat. No.: 8435

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: SRPK1 antibody was raised against a 19 amino acid peptide from near the amino terminus of human SRPK1.

The immunogen is located within the first 50 amino acids of SRPK1.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: SRPK1 antibody can be used for the detection of SRPK1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry at 5 μg/ml.

Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: SRPK1 antibody is human, mouse and rat reactive. Multiple isoforms of SRPK1 are known to exist; this antibody will detect all except isoforms b and e. SRPK1 antibody is predicted to not cross-react with SRPK2.
POSITIVE CONTROL:1) Cat. No. 1404 - Mouse Liver Tissue Lysate
 2) Cat. No. 11-701 - Human Testis Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 54, 72, 91 kDa

Observed: 91 kDa

psi-iconProperties
PURIFICATION:SRPK1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SRPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SRPK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:SRPK1
ALTERNATE NAMES:SRSF protein kinase 1, Serine/arginine-rich protein-specific kinase 1, Serine/threonine-protein kinase SRPK1, SFRSK1, SR-protein-specific kinase 1
ACCESSION NO.:NP_003128
PROTEIN GI NO.:47419936
GENE ID:6732
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SRPK1 and SRPK2, two Arginine/serine-rich (SR) protein-specific kinases constitute control circuits to regulate both constitutive and alternative pre-mRNA splicing and coordinate splicing with transcription in cells (1). They are highly specific for the phosphorylation of these RS proteins, and highly expressed in testis (1,2). SRPK1 plays a role in the condensation of sperm chromatin and controls the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis (3,4).
REFERENCES:1) Wang HY, Arden KC, Bermingham JR Jr, et al. Localization of serine kinases, SRPK1 (SFRSK1) and SRPK2 (SFRSK2), specific for the SR family of splicing factors in mouse and human chromosomes. Genomics 1999; 57:310-5.
2) Daub H, Blencke S, Habenberger P, et al. Identification of SRPK1 and SRPK2 as the major cellular protein kinases phosphorylating hepatitis B virus core protein. J. Virol. 2002; 76:8124-37.
3) Zhong XY, Ding JH, Adams JA, et al. Regulation of SR protein phosphorylation and alternative splicing by modulating kinetic interactions of SRPK1 with molecular chaperones. Genes Dev. 2009; 23:482-95.
4) Mavrou A, Brakspear K, Hamdollah-Zadeh M, et al. Serine-arginine protein kinase 1 (SRPK1) inhibition as a potential novel targeted therapeutic strategy in prostate cancer. Oncogene 2014 Nov 10. doi: 10.1038/onc.2014.360.

ANTIBODIES FOR RESEARCH USE ONLY.

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