SQSTM1 Antibody Cat. No.: 5449

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: SQSTM1 antibody was raised against a 13 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1.

The immunogen is located within the last 50 amino acids of SQSTM1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: WB: 0.5-2 μg/mL; IF: 20 μg/mL; IHC: 2-5 μg/mL;.

Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 1204 - K562 Cell Lysate
 3) Cat. No. 1201 – HeLa Cell Lysate
 4) Cat. No. 1203 – A549 Cell Lysate
 5) Cat. No. 1282 – 3T3/NIH Cell Lysate
PREDICTED MOLECULAR WEIGHT: Predicted: 48kD

Observed: 65 kD
psi-iconAdvanced Validation
VALIDATION:

Induced Expression Validation (Figure 2): Raw 264.7 cells were treated with LPS (0.3 μg/mL) for different time period (0-24 hrs). SQSTM1 expression detected by anit-SQSTM1 antibodies (5449) was induced by LPS treatment and the expression levels were increased gradually over the period of 24 hrs.

ISOFORMS: Human SQSTM1 has two isoforms, including isoform 1 (440aa, 47.7kD) and isoform 2 (356aa, 38.6kD). Mouse SQSTM1 also has two isoforms, including isoform 1 (442aa, 48.2kD) and isoform 2 (404aa, 44.2kD). Rat SQSTM1 has three isoforms, including isoform 1 (439aa, 47.7kD), isoform 2 (412aa, 45kD) and isoform 3 (234aa, 26.1kD). 5449 can detect the isoforms of human mouse and rat except the shortest rat isoform 3.

psi-iconProperties
PURIFICATION:SQSTM1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SQSTM1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SQSTM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:SQSTM1
ALTERNATE NAMES:SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP
ACCESSION NO.:Q13501
PROTEIN GI NO.:74735628
GENE ID:8878
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SQSTM1 Antibody: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-κB by TNF-α, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1's UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget's disease of bone since the UBA is necessary for aggregate sequestration and cell survival.
REFERENCES:1) Seibenhener et al. Mol. Cell. Biol.2004; 24:8055-68.
2) Hocking et al. Hum. Mol. Genet.2002; 11:2735-9.
3) Rousiere et al. Best Pract. Res. Clin. Rheumatol.2003; 17:1019-41.
4) Layfield et al. Biochem. Soc. Trans.2004; 32:728-30.

ANTIBODIES FOR RESEARCH USE ONLY.

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