SPDEF Antibody Cat. No.: 27-414

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPDEF.
TESTED APPLICATIONS: ELISA, WB
APPLICATIONS: SPDEF antibody can be used for detection of SPDEF by ELISA at 1:12500. SPDEF antibody can be used for detection of SPDEF by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
POSITIVE CONTROL:1) Cat. No. 1219 - MCF7 Cell Lysate
PREDICTED MOLECULAR WEIGHT: 38 kDa

psi-iconProperties
PURIFICATION:Antibody is purified by protein A chromatography method.
CLONALITY:Polyclonal
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
CONCENTRATION:batch dependent
STORAGE CONDITIONS:For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPDEF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:SPDEF
ALTERNATE NAMES:SPDEF, PDEF, RP11-375E1__A.3, bA375E1.3
ACCESSION NO.:NP_036523
PROTEIN GI NO.:6912580
GENE ID:25803
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA expression.PDEF is an ETS transcription factor expressed in prostate epithelial cells. It acts as an androgen-independent transactivator of PSA (MIM 176820) expression.
REFERENCES:1) Fowler, M., (2006) J. Cell. Biochem. 97 (1), 1-17.

ANTIBODIES FOR RESEARCH USE ONLY.

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