SPATA1 Antibody Cat. No.: 6549

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (72%)
IMMUNOGEN: SPATA1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPATA1.

The immunogen is located within amino acids 300 - 350 of SPATA1.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: SPATA1 antibody can be used for detection of SPATA1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
SPECIFICITY: At least two isoforms of SPATA1 are known to exist; this antibody will detect only the longest isoforms. SPATA1 antibody is predicted to not cross-react with other SPATA family members.
POSITIVE CONTROL:1) Cat. No. 1288 - A20 Cell Lysate

psi-iconProperties
PURIFICATION:SPATA1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SPATA1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SPATA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:SPATA1
ALTERNATE NAMES:SPATA1 Antibody: SP-2, SPAP1, Spermatogenesis-associated protein 1, Sperm-specific protein SP-2
ACCESSION NO.:Q5VX52
PROTEIN GI NO.:341915102
GENE ID:100505741
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SPATA1 Antibody: SPATA1, a novel cell-surface protein, is involved in shaping the sperm head during spermatogenesis. SPATA1 is localized at chromosome 1q21. At least three isoforms of SPATA1 are known to exist. The longest is preferentially expressed in hematopoietic tissues. Its extracellular domain contains a single immunoglobulin-like domain, and its intracellular segment has two immunoreceptor tyrosine-based inhibition motifs (ITIMs). It is thought to have an important role in hematopoietic cell signaling. The mid-sized isoform contains a short intracellular part without ITIMs, while the shortest isoform lacks the transmembrane segment and represents a potential soluble protein (2, 3).
REFERENCES:1) Giesecke K, Hamann H, Stock KF, et al. Evaluation of SPATA1-associated markers for stallion fertility. Anim. Genet. 2009; 40:359-65.
2) Xu MJ, Zhao R, Zhao ZJ. Molecular cloning and characterization of SPAP1, an inhibitory receptor. Biochem. Biophys. Res. Commun. 2001; 280:768-75
3) Liu SF, He S, Liu BW, et al. Cloning and characterization of testis-specific spermatogenesis associated gene homologous to human SPATA4 in rat. Biol. Pharm. Bull. 2004; 27:1867-70.

ANTIBODIES FOR RESEARCH USE ONLY.

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