Datasheet

SOX10 Antibody
CATALOG NUMBER: 8481

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
HOMOLOGY:Predicted species reactivity based on immunogen sequence: Pig: (100%), Chicken: (89%), Goat: (100%)
TESTED APPLICATIONS:ELISA, IF, IHC-P, WB
APPLICATIONS:SOX10 antibody can be used for Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1461 - Rat Heart Tissue Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 51 kDa

Observed: 54 kDa
SPECIFICITY:SOX10 Antibody is predicted to not cross-react with other SOX familiy members.
IMMUNOGEN:SOX10 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human SOX10.
HOST SPECIES:Rabbit

Properties

PURIFICATION:SOX10 Antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:SOX10 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SOX10 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:SOX10 Antibody: SRY (sex determining region Y)-box 10, DOM,WS4, PCWH, WS2E, WS4C
ACCESSION NO.:NP_008872
PROTEIN GI NO.:5902104
OFFICIAL SYMBOL:SOX10
GENE ID:6663

Background

BACKGROUND:SOX10 Antibody: SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and the determination of the cell fate (1). The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins (2). This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development (2). Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschprung disease (3). SLC30A8 expression in beta-cells was found to be influenced by cytokine expression, particularly IFN-gamma and IL-1beta (4).
REFERENCES: 1) Pusch C, Hustert E, Pfeifer D, et al. The SOX10/Sox10 gene from human and mouse: sequence, expression, and transactivation by the encoded HMG domain transcription factor. Hum. Genet. 1998; 103:115-23.
2) Rehberg S, Lischka P, Glaser G, et al. Sox10 is an active nucleocytoplasmic shuttle protein, and shuttling is crucial for Sox10-mediated transactivation. Mol. Cell Biol. 2002; 22:5826-34.
3) Pingault V, Bondurand N, Kuhlbrodt K, et al. SOX10 mutations in patients with Waardenburg-Hirschprung disease. Nat. Genet. 1998; 18:171-3.

For Research Use Only