Datasheet

SMURF1 Antibody
CATALOG NUMBER: 7945

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Specifications
Properties
Additional Info
Background

Specifications

SPECIES REACTIVITY:Human, Mouse, Rat
TESTED APPLICATIONS:ELISA, IHC-P, WB
APPLICATIONS:SMURF1 body can be used for detection of SMURF1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in rat samples. All other applications and species not yet tested.
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
PREDICTED MOLECULAR WEIGHT:Predicted: 80, 83 kDa

Observed: 78 kDa
SPECIFICITY:SMURF1 antibody is human, mouse and rat reactive. At least three isoforms of SMURF1 are known to exist this antibody will recognize all three isoforms. SMURF1 is predicted to not cross-react with SMURF2.
IMMUNOGEN:SMURF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human SMURF1.

The immunogen is located within amino acids 180 - 230 of SMURF1.
HOST SPECIES:Rabbit

Properties

PURIFICATION:SMURF1 antibody is affinity chromatography purified via peptide column.
PHYSICAL STATE:Liquid
BUFFER:SMURF1 antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SMURF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated

Additional Info

ALTERNATE NAMES:SMAD specific E3 ubiquitin protein ligase 1, SMAD ubiquitination regulatory factor 1, E3 ubiquitin-protein ligase SMURF1
ACCESSION NO.:NP_065162
PROTEIN GI NO.:31317292
OFFICIAL SYMBOL:SMURF1
GENE ID:57154

Background

BACKGROUND:SMURF1 (SMAD ubiquitin regulatory factor 1) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF1 negatively regulates osteoblast activity and response to bone morphogenesis protein (BMP) through controlling MEKK2 degradation (3). SMURF1 has recently been shown to play a role in breast cancer cell migration and invasion through the downregulation of RhoA (4).
REFERENCES: 1) Huibregtse JM, Scheffner M, Beaudenon S, et al. A family of proteins structurally and functionally related to the E6-AP ubiquitin-protein ligase. Proc. Natl. Acad. Sci. USA 1995; 92:2563-7.
2) Hwang YS, Lee HS, Kamata T, et al. The Smurf ubiquitin ligases regulate tissue separation via antagonistic interactions with ephrinB1. Genes Dev. 2013; 27:491-503.
3) Yamashita M, Ying SX, Zhang G, et al. Ubiquitin ligase Smurf1 controls osteoblast activity and bone homeostasis by targeting MEKK2 for degradation. Cell 2005; 121:101-113.
4) Kwon A, Lee HL, Woo KM, et al. SMURF1 plays a role in EGF-induced breast cancer cell migration and invasion. Mol. Cells 2013; 36:548-55.

For Research Use Only