Smad2 Antibody [6D1B1] Cat. No.: 13-174

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psi-iconSpecifications
HOST SPECIES:Mouse
SPECIES REACTIVITY: Human
IMMUNOGEN: Recombinant protein of human Smad2
TESTED APPLICATIONS: ICC, WB
APPLICATIONS: WB: ,1:500 - 1:2000

ICC: ,1:50 - 1:200
POSITIVE CONTROL:1) HeLa
 2) 293T
 3) THP-1
 4) Mouse pancreas
PREDICTED MOLECULAR WEIGHT: Observed: 50kDa

psi-iconProperties
PURIFICATION:Affinity purification
CLONALITY:Monoclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS:Store at -20˚C. Avoid freeze / thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:SMAD2
ALTERNATE NAMES:SMAD2, JV18, MADH2, MADR2, JV18-1, hMAD-2, hSMAD2
GENE ID:4087
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.

ANTIBODIES FOR RESEARCH USE ONLY.

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