|SOURCE:||Human Skin tissue|
|APPLICATIONS:||Skin membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.|
|BUFFER:||HEPES pH 7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, and NP-40.|
|CONCENTRATION:||2 mg/mL or better|
|STORAGE CONDITIONS:||Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.|
|Human skin tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human skin tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated skin tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated skin tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.|
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For Research Use Only