SKI2W Antibody Cat. No.: 7993

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
IMMUNOGEN: SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W.

The immunogen is located within amino acids 60 - 110 of SKI2W.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
APPLICATIONS: SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms.
POSITIVE CONTROL:1) Cat. No. 1415 - Mouse Stomach Tissue Lysate
 2) Cat. No. 10-801 - Human Stomach Tissue Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 113, 137 kDa

Observed: 135 kDa

psi-iconProperties
PURIFICATION:SKI2W antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SKI2W antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SKI2W antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

psi-iconAdditional Info
OFFICIAL SYMBOL:SKIV2L
ALTERNATE NAMES:Superkiller viralicidic activity 2-like, DDX13, Helicase-like protein, Helicase SKI2W, HLP, SKI2, SKIV2, SKIV2L
ACCESSION NO.:NP_008860
PROTEIN GI NO.:67782311
GENE ID:6499
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SKI2W (SKIV2L, Helicase-like protein), a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1,2). SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2). The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3,4).
REFERENCES:1) Dangel AW, Shen L, Mendoza AR, et al. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 1995; 23:2120-6.
2) Qu X, Yang Z, Zhang S, et al. The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes. Nucleic Acids Res. 1998; 26:4068-77.
3) Yang Z, Shen L, Dangel AW, et al. Four ubiquitously expressed genes, RD (D6S45)-SKI2W (SKIV2L)-DOM3Z-RP1 (D6S60E), are present between complement component genes factor B and C4 in the class III region of the HLA. Genomics 1998; 53:338-47.
4) Yang Z, Qu X, and Yu CY. Features of the two gene pairs RD-SKI2W and DOM3Z-RP1 located between complement component genes factor B and C4 at the MHC class III region. Front. Biosci. 2001; 6:D927-35.

ANTIBODIES FOR RESEARCH USE ONLY.

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