Single Domain Antibodies
Single domain antibodies (sdAbs), also known as VHH antibodies, are recombinant antibodies comprised of the binding domain from heavy chain only camelid antibodies. Due to their small size, ~15kDa, and their longer and structurally unique Complementarity Determining Region 3 (CDR3 region), they target unique epitopes inaccessible by conventional antibodies. Other benefits of sdAbs include enhanced stability at wider temperature and pH ranges, making them functional in more applications than traditional antibodies. Read more on our blog!
ProSci takes great care during the development process to select high-performance antibodies. Starting with immunization of proteins expressed in appropriate systems (e.g. mammalian systems for human proteins) to generate an ideal immune response, a cDNA library is constructed and then panned in solution for binders. These binders are then extensively screened for a variety of applications, including ELISA, histology, and flow cytometry.
Note: Due to the unique characteristics of sdAbs, they rarely characterize well for applications such as Western Blotting.
So how are single-domain antibodies detected if they do not have a constant region? ProSci sdAbs are easy to detect with ProSci Cat. No. PM-7669.
Recombinantly produced, sdAbs allow for lot-to-lot reproducibility, maintaining consistency of quality. These antibodies are also easily genetically manipulated and the binding domain can be fused to other scaffolds such as Fc domains.
Contact ProSci if you are interested in engineering the current catalog of ProSci single domain antibodies for your proof-of-concept experiments or licensing opportunities.