SIK1 Antibody Cat. No.: 6299

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (73%), Mouse: (73%)
IMMUNOGEN: SIK1 antibody was raised against an 18 amino acid synthetic peptide near the center of human SIK1.

The immunogen is located within amino acids 340 - 390 of SIK1.
TESTED APPLICATIONS: ELISA, IHC-P, WB
APPLICATIONS: SIK1 antibody can be used for detection of SIK1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.

Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
SPECIFICITY: Two isoforms of SIK1 are known to exist; this antibody will detect both isoforms. SIK1 antibody is predicted to not cross-react with other SIK protein family members.
POSITIVE CONTROL:1) Cat. No. 1308 - Human Small Intestine Tissue Lysate
 2) Cat. No. 11-801 - Human Small Intestine Tissue Slide

psi-iconProperties
PURIFICATION:SIK1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SIK1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SIK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:SIK1
ALTERNATE NAMES:SIK1 Antibody: MSK, SIK, SNF1LK, Salt-inducible kinase 1, SIK-1
ACCESSION NO.:NP_775490
PROTEIN GI NO.:116256471
GENE ID:150094
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SIK1 Antibody: Salt-inducible kinase 1 (SIK1), also known as SNF1LK or MSK, plays a role in histone modification and G2/M cell cycle regulation. It is a 783 amino acid protein that contains one UBA domain and belongs to the Ser/Thr protein kinase family (AMPK subfamily). Localized to both the nucleus and the cytoplasm, SIK1 is a class II HDAC kinase that uses magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of target proteins and is thought to be important for the early stages of skeletal muscle growth and myocardial cell differentiation.
REFERENCES:1) Stephenson A, Huang GY, Nguyen NT, et al. SNF1LK encodes a protein kinase that may function in cell cycle regulation. Genomics 2004; 83:1105-15
2) Ruiz JC, Conlon FL, and Robertson EJ. Identification of novel protein kinases expressed in the myocardium of the developing mouse heart. Mech. Dev. 1994; 48:153-64
3) Lizcano JM, Goransson O, Toth R, et al. LKB1 is a master kinase that activates 13 kinases of the AMPK subfamily, including MARK/PAR-1. EMBO J. 2004; 23:833-43.
4) Sjöström M, Senstrom K, Eneling K, et al. SIK1 is part of a cell sodium-sensing network that regulates active sodium transport through a calcium-dependent process. Proc. Natl. Acad. Sci. USA 2007; 104:16922-7.

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