Biological activity was determined by its ability to inhibit BMP - 2 proliferation effect of ATDC - 5 cells. The expected ED50is 0.3-0.5 μg/mL.
Properties
PURITY:
Greater than 97% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg).
PHYSICAL STATE:
Lyophilized
STORAGE CONDITIONS:
The lyophilized Secreted FRP-1 recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted Secreted FRP-1 is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing Secreted FRP-1 recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Additional Info
ALTERNATE NAMES:
FRP, FRP1, FrzA, FRP-1, SARP2, FRP, Secreted frizzled-related protein 1, Secreted apoptosis-related protein 2
ACCESSION NO.:
NP_003003.3
PROTEIN GI NO.:
56117838
OFFICIAL SYMBOL:
SFRP1
GENE ID:
6422
Background and References
BACKGROUND:
Secreted Frizzled Related Proteins (sFRPs) modulate WNT signaling by binding directly to WNT proteins in a manner that affects their receptor binding and signaling capabilities. sFRP-1 is a widely distributed protein that can bind directly to WNT1, WNT2, and possibly other WNT proteins, and generally exerts anti-proliferative effects consistent with activity as a WNT antagonist. It also inhibits apoptosis and has been found to be down-regulated in many solid tumors, but up-regulated in uterine leiomyomas. Recombinant human sFRP-1 is a glycosylated 283 amino acid protein containing a cysteine rich Frizzled homologous domain.
Specifications
