SCARA5 Antibody Cat. No.: 5259

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Mouse: (88%), Chicken: (86%), Rat: (80%)
IMMUNOGEN: SCARA5 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SCARA5.

The immunogen is located within amino acids 350 - 400 of SCARA5.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: SCARA5 antibody can be used for detection of SCARA5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: At least four isoforms of SCARA5 are known to exist; this antibody will detect all four isoforms.
POSITIVE CONTROL:1) Cat. No. 1306 - Human Spleen Tissue Lysate
 2) Cat. No. 17-011 - Hep G2 Cell Slide
PREDICTED MOLECULAR WEIGHT: Predicted: 24, 39, 44, 54 kDa

Observed: 50 kDa

psi-iconProperties
PURIFICATION:SCARA5 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SCARA5 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SCARA5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:SCARA5
ALTERNATE NAMES:SCARA5 Antibody: Tesr, NET33, Scavenger receptor class A member 5, Scavenger receptor hlg
ACCESSION NO.:NP_776194
PROTEIN GI NO.:47271477
GENE ID:286133
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:SCARA5 Antibody: Scavenger receptor class A member 5 (SCARA5), also known as TESR, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARA5 is a cell-surface receptor that contains the collagen-like domain and N-terminal scavenger receptor cysteine rich domain typical of the class A scavenger receptor subfamily. Unlike other class A receptors, SCARA5 does not endocytose acetylated or oxidized low density lipoprotein. Instead, SCARA5 binds serum ferritin and mediates its endocytosis from the cell surface, indicating SCARA5 may play a role in non-transferrin iron delivery.
REFERENCES:1) Greaves DR, Gough PJ, and Gordon S. Recent progress in defining the role of scavenger receptors in lipid transport, atherosclerosis and host defence. Curr. Op. Lipid. 1998; 9:425-32.
2) Jiang Y, Oliver P, Davies KE, et al. Identification and characterization of murine SCARA5, a novel class A scavenger receptor that is expressed by populations of epithelial cells. J. Biol. Chem. 2006; 281:11834-45.
3) Li JY, Paragas N, Ned RM, et al. Scara5 is a ferritin receptor mediating non-transferrin iron delivery. Dev. Cell 2009; 16:35-46.

ANTIBODIES FOR RESEARCH USE ONLY.

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