Specifications
| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: |
Virus |
| IMMUNOGEN: |
SARS envelope antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of the SARS envelope protein.
The immunogen is located within the last 50 amino acids of SARS Envelope. |
| TESTED APPLICATIONS: |
ELISA |
| APPLICATIONS: |
SARS envelope antibody can be used for the detection of SARS envelope protein in ELISA. It will detect 10 ng of free peptide at 1 μg/mL. |
Properties
| PURIFICATION: | SARS Envelope Antibody is affinity chromatography purified via peptide column. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | SARS Envelope Antibody is supplied in PBS containing 0.02% sodium azide. |
| CONCENTRATION: | 1 mg/mL |
| STORAGE CONDITIONS: | SARS Envelope antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Additional Info
| OFFICIAL SYMBOL: | E |
| ALTERNATE NAMES: | SARS Envelope Antibody: sM, 4, Envelope small membrane protein, E protein |
| ACCESSION NO.: | P59637 |
| PROTEIN GI NO.: | 30173401 |
| GENE ID: | 1489671 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher. |
Background and References
| BACKGROUND: | SARS Envelope Antibody: A novel coronavirus has recently been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive-stranded RNA approximately 27-31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin-converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2. |
| REFERENCES: | 1) Marra MA, Jones SJ, Astell CR, et al. The Genome sequence of the SARS-associated corona virus. Science 2003;300:1399-404. |
| 2) Rota PA, Oberste MS, Monroe SS, et al. Characterization of a novel coronavirus associated with severe acute respiratory syndrome. Science 2003;300:1394-9. | | 3) Navas-Nartin SR and Weiss S. Coronavirus replication and pathogenesis: Implications for the recent outbreak of severe acute respiratory syndrome (SARS), and the challenge for vaccine development. J Neurovirol. 2004;10:75-85. | | 4) Arbely E, Khattari Z, Brotons G, et al. A highly unusual palindromic transmembrane helical hairpin formed by SARS coronavirus E protein. J Mol. Biol. 2004;3414:769-79. |
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