SARS-CoV-2 Spike NTD Antibody [2146] (Biotin) Cat. No.: 10-056

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psi-iconSpecifications
SPECIES REACTIVITY: Virus
IMMUNOGEN: Sequenced from human survivors of COVID-19 (SARS-CoV-2). The spike NTD is expressed on the surface of SARS-CoV-2.
TESTED APPLICATIONS: ELISA, IHC
APPLICATIONS: ELISA (Quality Tested by ProSci); IHC (Other applications as reported in literature)
SPECIFICITY: Anti-SARS-CoV-2 Spike NTD-Biotin, clone 2146, specifically targets an epitope on the SARS-CoV-2 spike protein N-terminal domain. This antibody does not cross-react with the receptor-binding domain (RBD) of the spike protein or the NTD of SARS-CoV.

psi-iconProperties
CLONALITY:Monoclonal
ISOTYPE:Human IgG1
CONJUGATE:Biotin
PHYSICAL STATE:Liquid
BUFFER:This biotinylated monoclonal antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
CONCENTRATION:0.5 mg/ml
STORAGE CONDITIONS:This biotinylated monoclonal antibody is stable when stored at 2-8˚ C. Do not freeze.Thaw Cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:S
ALTERNATE NAMES:SARS-CoV2 Spike NTD, COVID-19, 2019-nCoV, Severe acute respiratory syndrome coronavirus 2, SARS-CoV2
PROTEIN GI NO.:SARS-CoV-2
USER NOTE:43740568
psi-iconBackground and References
REFERENCES:1) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), is an enveloped, single-stranded, positive-sense RNA virus that belongs to the Coronaviridae family 1. The SARS-CoV-2 genome, which shares 79.6% identity with SARS-CoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) 2. The S protein is a transmembrane, homotrimeric, class I fusion glycoprotein that mediates viral attachment, fusion, and entry into host cells 3. Each ~180 kDa monomer contains two functional subunits, S1 (~700 a.a) and S2 (~600 a.a), that mediate viral attachment and membrane fusion, respectively. S1 contains two major domains, the N-terminal (NTD) and C-terminal domains (CTD). In both SARS-CoV and SARS-CoV-2, the CTD contains the receptor-binding domain (RBD), which binds to the angiotensin-converting enzyme 2 (ACE2) receptor on host cells3-5. The NTD of SARS-CoV-2 does not bind to ACE26, and the function of NTD in SARS-CoV-2 infection is not well understood. In other CoVs, the NTD may promote attachment by binding to sugar moieties7 and might play a role in the conformational change of S2 required for membrane fusion8. While most neutralizing antibodies target the RBD domain and block receptor binding, potent neutralizing antibodies targeting NTD were isolated from convalescent COVID19 patients9, identifying the NTD as an attractive candidate for vaccines and therapeutics. In addition, the NTD is a promising antigen for diagnostic tests, as there is only 53.5% homology between the NTD of SARS-CoV-2 and SARS-CoV10.
2) Zhou, P., Yang, X., Wang, X. et al. Nature 579, 270–273. 2020.
3) Wu, F., Zhao, S., Yu, B. et al. Nature 579, 265–269. 2020.
4) Wrapp D, Wang N, Corbett KS, et al. bioRxiv. 2020.
5) Walls AC, Park YJ, Tortorici MA, et al. Cell. 181(2):281-292.e6. 2020.
6) Li W, Zhang C, Sui J, et al. EMBO J. 24(8):1634-1643. 2020.
7) Wang Q, Zhang Y, Wu L, et al. Cell. 181(4):894-904.e9. 2020.
8) Park, Y., Walls, A.C., Wang, Z. et al. Nat Struct Mol Biol 26, 1151–1157. 2019.
9) Zhou, H., Chen, Y., Zhang, S. et al. Nat Commun 10, 3068. 2019.
10) Chi X, Yan R, Zhang J, et al. Science. eabc6952. 2020.
psi-iconCITATIONS
CITATIONS: 1)Ou, X., Liu, Y., Lei, X. et al. Nat Commun 11, 1620. 2020.

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