SARS CoV-2 full length spike protein in DIBMA Glycerol Cat. No.: 21-816

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psi-iconSpecifications
SPECIES:SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Protein
SOURCE SPECIES:HEK293 cells
SEQUENCE:Full-length sequence (aa 1 – 1273),
furin cleavage site “RRAR” mutated to “GSAG”;
C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
FUSION TAG:C-terminal Rho1D4
TESTED APPLICATIONS:ELISA
APPLICATIONS:This recombinant protein can be used for ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses. For research use only.
BIOLOGICAL ACTIVITY: not determined
psi-iconProperties
PURITY:>98% as determined by SDS-PAGE, see Fig. 1 A and B. PureCube Rho1D4 Agarose.
PREDICTED MOLECULAR WEIGHT:1286 amino acids (including Rho1D4 tag and linker); 142114 Da
PHYSICAL STATE:Liquid
BUFFER:20 mM Hepes pH 7.5; 150 mM NaCl,: buffer does not contain free DIBMA Glycerol (DIBMA: Diisobutylene-maleic acid)
CONCENTRATION:batch dependent
STORAGE CONDITIONS:–80˚C. Avoid freeze-thaw cycles
psi-iconAdditional Info
ALTERNATE NAMES:SARS-CoV-2 (COVID-19) Spike Recombinant Protein: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
OFFICIAL SYMBOL:S
GENE ID:43740568
psi-iconBackground and References
BACKGROUND:The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor. The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways: MSP nanodiscs, based on MSP proteins Detergent Mycelles, as you can see here Synthetic nanodiscs

FOR RESEARCH USE ONLY.

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