SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody Cat. No.: 9187

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Virus
HOMOLOGY: Predicted reactivity based on immunogen sequence: SARS-CoV NSP14 (ExoN) protein: (79%).
IMMUNOGEN: Anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (9187) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein.

The immunogen is located between 30-80 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN).
TESTED APPLICATIONS: ELISA
APPLICATIONS: Antibody validated: SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA.

psi-iconProperties
PURIFICATION:SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ExoN
ALTERNATE NAMES:Proofreading exoribonuclease, Guanine-N7 methyltransferase, Non-structural protein 14, NSP14.
ACCESSION NO.:YP_009725309.1
PROTEIN GI NO.:1802476817
GENE ID:43740578
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP14 (ExoN) is an enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (3,4,5). It acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens(6,7,8).
REFERENCES:1) Gorbalenya. bioRxiv: 2020.
2) Hui et al. Int J Infect Dis. 2020;91:264-266.
3) Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113
4) Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863
5) Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377
6) Denison et. al. RNA Biol. 2011;8:270-279
7) Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565
8) Agostini et. al. MBio 2018; 9:0-0

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