This recombinant protein can be used for WB. For research use only.
Properties
PURITY:
>90% as determined by SDS-PAGE.
PREDICTED MOLECULAR WEIGHT:
22-25 kDa
PHYSICAL STATE:
Lyophilized
BUFFER:
PBS, Arginine, pH7.4, trehalose is added as protectant before lyophilization.
STORAGE CONDITIONS:
Lyophilized protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -70˚C or lower. Avoid repeated freeze-thaw cycles.
The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
Specifications
