The E. coli derived 35 kDa recombinant protein contains Nucleocapsid protein 1-49, 192-220 amino acids immunodominant regions. The 2 regions are separated with 3 glycine residues.
TESTED APPLICATIONS:
ELISA, WB
APPLICATIONS:
This recombinant protein can be used for WB, ELISA.
BIOLOGICAL ACTIVITY:
N/A
Properties
PURITY:
>95% pure as determined by 10% PAGE (coomassie staining). Purified by proprietary chromatographic technique.
PHYSICAL STATE:
Liquid
BUFFER:
50mM Tris-HCl, pH-8, 60mM NaCl and 50% glycerol.
STORAGE CONDITIONS:
Store in working aliquots at -20˚C. Avoid freeze/thaw cycles.
Background and References
BACKGROUND:
SARS Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. Spike (S)-glycoprotein of the virus interacts with a cellular receptor and mediates membrane fusion to allow viral entry into susceptible target cells. Accordingly, S-protein plays an important role in virus infection cycle and is the primary target of neutralizing antibodies.
Specifications
