ROCK1 Antibody Cat. No.: 6295

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rabbit: (93%)
IMMUNOGEN: ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1.

The immunogen is located within the first 50 amino acids of ROCK1.
TESTED APPLICATIONS: ELISA, ICC, IF, WB
APPLICATIONS: ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.

Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
SPECIFICITY: ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
POSITIVE CONTROL:1) Cat. No. 1210 - HEK293 Cell Lysate
 2) Cat. No. 17-010 - HEK293 Cell Slide

psi-iconProperties
PURIFICATION:ROCK1 Antibody is affinity chromatography purified via peptide column.
CLONALITY:Polyclonal
ISOTYPE:IgG
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:ROCK1 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION:1 mg/mL
STORAGE CONDITIONS:ROCK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

psi-iconAdditional Info
OFFICIAL SYMBOL:ROCK1
ALTERNATE NAMES:ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, ROCK-I
ACCESSION NO.:NP_005397
PROTEIN GI NO.:4885583
GENE ID:6093
USER NOTE:Optimal dilutions for each application to be determined by the researcher.
psi-iconBackground and References
BACKGROUND:ROCK1 Antibody: ROCK1 is a member of the AGC serine/threonine protein kinase family that regulates the assembly of the actin cytoskeleton. ROCK1 contains a protein kinase domain, a coiled-coil region and a zinc finger region and appears to be present as a dimer. ROCK1, like its isozyme ROCK2, is a downstream target of the small G-protein Rho and plays a role in smooth muscle contraction, malignant cell transformation, tumor invasion and metastasis, and mediates the cellular morphological changes during apoptosis.
REFERENCES:1) Ishizaki T, Maekawa M, Fujisawa K, et al. The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. EMBO J. 1996; 15:1885-93.
2) Takahashi N, Tuiki H, Saya H, et al. Localization of the gene coding for ROCK II/Rho kinase on human chromosome 2p24. Genomics 1999; 55:235-7.
3) Leung T, Chen XQ, Manser E, et al. The p160 RhoA-binding kinase ROK alpha is a member of a kinase family and is involved in the reorganization of the cytoskeleton. Mol. Cell Biol. 1996; 16:5313-27.

ANTIBODIES FOR RESEARCH USE ONLY.

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