Risk-Free™ Immune Checkpoint Products

What is Risk-Free™?

In a 2008 study, fewer than half of over 6,000 commercially available antibodies recognized only their targets [1,2], leading to an estimated $350 million wasted on "bad" antibodies [1]. ProSci Inc. is proud to announce the release of their new line of Risk-Free™ antibodies.

ProSci's Risk-Free™ antibodies are monoclonal antibodies made to improve in vitro studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-Free™ antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. These antibodies have the following advantages:

  • Antibodies are developed against mammalian-expressed, post-translationally modified recombinant proteins.
  • Multiple antibodies per target allows the user to choose the best antibody for their application
  • Available individually or as a set
  • Risk-Free™ means they are rigorously tested and guaranteed to work for their designated applications:
    • • ELISA• Flow Cytometry• Immunoblot • Immunohistochemistry • Immunocytochemistry • Immunofluorescence

Risk-Free™ Immune Checkpoint Antibody Sets

Many antibodies work well one application but not in others despite detecting the same proteins. Because the target antigen may be denatured or inaccessible depending on the technique or preparation, scientists must search and test multiple antibodies for one application and repeat the process again for a different application. ProSci has recognized this issue and has released Risk-Free™ Antibody Sets.


Risk-Free™ Immune Checkpoint Targets

ProSci PD-1

PD-1


The PD-1 protein is a negative co-stimulatory receptor that can bind two ligands, PD-L1 and PD-L2. Upon binding, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals."

Host Species:
Mouse PD-1 Llama PD-1

ProSci PD-L1

PD-L1


PD-L1 is a B7-related protein that inhibits cell-mediated immune responses by reducing the secretion of IL-2 and IL-10 from memory T cells. Upon binding with PD-1, signals generated by PD-1 inhibit the activation of the immune response.

Host Species:
Mouse PD-L1 Mouse PD-L1

CTLA-4

CTLA-4


The CTLA-4 protein, also known as CD152, transmits an inhibitory signal to T cells. Both it and the homologous T-cell
co-stimulatory protein CD28 bind to CD80 (B7-H1) and CD86 (B7-H2) on
antigen-presenting cells (APCs).

Host Species:
Mouse CTLA-4

 

ProSci PD-L2

PD-L2


PD-L2 is a negative regulator of T-cell activation. PD-L2 can bind to PD-1, which mediates inhbiotry signals from the APC. However, it has been suggested that PD-L2 can act to stimulate an immunogenic response through an alternative receptor from PD-1.

Host Species:
Mouse PD-L2

ProSci CD-80

CD-80


CD80 is a member of the immunoglobulin superfamily that is expressed by antigen-presenting cells, and is the ligand for CD28 and CTLA-4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell and induces T-cell proliferation and cytokine production.

Host Species:
Mouse CD-80

TIGIT

TIGIT


TIGIT interacts with CD155, and mediates the interaction between natural killer cells or T lymphocytes with APC and fibroblasts and endothelial cells expressing PVR or PVR-like proteins.

Host Species:
Mouse TIGIT

 

ProSci LIGHT

LIGHT


LIGHT (CD258) is a type II membrane protein belonging to the TNF family of membrane-anchored ligands. LIGHT has the ability to activate NFkB, stimulate proliferation of lymphocytes, and induce apoptosis in certain human tumor cells.

Host Species:
Mouse LIGHT

 

References

1. Berglund L, Bjorling E, Oksvold P, et al. "A gene-centric human protein atlas for expression profiles based on antibodies."Mol. Cell Proteomics 2008; 7:2019-27.

2. Bradbury A, Plucktun, et al. "Standardize antibodies used in research."Nature 2015; 518:27-9.