Rhesus macaque CD40 Ligand / TNFSF5 Recombinant Protein Cat. No.: 11-500

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psi-iconSpecifications
SPECIES:Human, Rhesus macaque
SOURCE SPECIES:HEK293 cells
SEQUENCE:Met 113 - Leu 261
FUSION TAG:Mouse IgG Fc Tag
TESTED APPLICATIONS:ELISA, WB
APPLICATIONS:This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 43.1 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
psi-iconProperties
PURITY:>95% as determined by SDS-PAGE.
PREDICTED MOLECULAR WEIGHT:43.1 kDa
PHYSICAL STATE:Lyophilized
BUFFER:50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
STORAGE CONDITIONS:Lyophilized Protein should be stored at -20˚C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20˚C or -70˚C. Avoid repeated freeze-thaw cycles.
psi-iconAdditional Info
ALTERNATE NAMES:CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
ACCESSION NO.:NP_000065.1
OFFICIAL SYMBOL:CD40LG
GENE ID:959
psi-iconBackground and References
BACKGROUND:CD40 ligand is also known as CD40L, CD154, TNFSF5 and T-cell antigen Gp39, is a single-pass type I I membrane protein which belongs to the TNF superfamily of molecules. CD40 ligand is expressed predominantly on activated CD4+ T lymphocytes, and also found in other types of cells, including platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, as well as non-haematopoietic cells (smooth muscle cells, endothelial cells, and epithelial cells). Although all monomeric, dimeric and trimeric forms of soluble CD40 ligand can bind to CD40, the trimeric form of soluble CD40 ligand has the most potent biological activity through oligomerization of cell surface CD40, a common feature of TNF receptor family members. CD40 ligand binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In general, CD40 ligand plays the role of a costimulatory molecule and induces activation in APC in association with T cell receptor stimulation by MHC molecules on the APC. In total CD40 ligand has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD40 ligand regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper IgM syndrome.
REFERENCES:1) Schönbeck U, Libby P, 2001, Cell. Mol. Life Sci. 58 : 4–43.
2) Furman M.I. et al., 2004, J. Am. Coll. Cardiol. 43: 2319-25.
3) Spriggs, M.K. et al., 1992, J. Exp. Med. 176:1543.
4) Fanslow, W.C. et al., 1994, Seminars in Immunology 6:267. 

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