Recombinant NKX2.2 Antibody [NX2/1422R] Cat. No.: 34-086

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psi-iconSpecifications
HOST SPECIES:Rabbit
SPECIES REACTIVITY: Human
IMMUNOGEN: A human partial recombinant protein was used as the immunogen for this recombinant NKX2.2 antibody.
TESTED APPLICATIONS: Flow, IF, IHC-P
APPLICATIONS: Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml

IF: 1-2 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 minutes at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the recombinant NKX2.2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.


psi-iconProperties
PURIFICATION:Protein A affinity chromatography
CLONALITY:Monoclonal
ISOTYPE:IgG, k
CONJUGATE:Unconjugated
PHYSICAL STATE:Liquid
BUFFER:PBS with 0.1 mg/ml BSA and 0.05% sodium azide
CONCENTRATION:0.2 mg/mL
STORAGE CONDITIONS:Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

psi-iconAdditional Info
OFFICIAL SYMBOL:NKX2-2
ALTERNATE NAMES:NKX2-2 Antibody: NKX2B, NKX2.2, NKX2B, Homeobox protein NK-2 homolog B
GENE ID:4821
USER NOTE:Optimal dilutions for each application to be determined by the researcher
psi-iconBackground and References
BACKGROUND:Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. Antibody to NKX2.2 detects a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.

ANTIBODIES FOR RESEARCH USE ONLY.

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