| HOST SPECIES: | Rabbit |
| SPECIES REACTIVITY: | Human |
| IMMUNOGEN: | Human Ig Gamma Chain was used as the immunogen for this recombinant IgG antibody. |
| TESTED APPLICATIONS: | Flow, IF, IHC-P, WB |
| APPLICATIONS: | Flow Cytometry: 0.5-1 ug/million cells in 0.1ml Immunofluorescence: 0.5-1 ug/ml Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT Prediluted IHC only format: incubate for 30 min at RT (1) The optimal dilution of the recombinant IgG antibody for each application should be determined by the researcher. 1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Specifications| PURIFICATION: | Protein A affinity chromatography |
| CLONALITY: | Monoclonal |
| ISOTYPE: | IgG, kappa |
| CONJUGATE: | Unconjugated |
| PHYSICAL STATE: | Liquid |
| BUFFER: | PBS with 0.1 mg/ml BSA and 0.05% sodium azide |
| CONCENTRATION: | 0.2 mg/mL |
| STORAGE CONDITIONS: | Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles. |
| OFFICIAL SYMBOL: | IGHG1 |
| GENE ID: | 3500 |
| USER NOTE: | Optimal dilutions for each application to be determined by the researcher |
| BACKGROUND: | Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It reacts with all sub-classes of gamma chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. |
ANTIBODIES FOR RESEARCH USE ONLY.
For additional information, visit ProSci's Terms & Conditions Page.
